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Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars

Common bean (Phaseolus vulgaris L.) is a major source of proteins and one of the most important edible foods for more than three hundred million people in the world. The common bean plants are frequently attacked by spider mite (Tetranychus urticae Koch), leading to a significant decrease in plant g...

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Autores principales: Hoseinzadeh, Abdul Hadi, Soorni, Aboozar, Shoorooei, Marie, Torkzadeh Mahani, Masoud, Maali Amiri, Reza, Allahyari, Hossein, Mohammadi, Rahmat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999899/
https://www.ncbi.nlm.nih.gov/pubmed/32017794
http://dx.doi.org/10.1371/journal.pone.0228680
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author Hoseinzadeh, Abdul Hadi
Soorni, Aboozar
Shoorooei, Marie
Torkzadeh Mahani, Masoud
Maali Amiri, Reza
Allahyari, Hossein
Mohammadi, Rahmat
author_facet Hoseinzadeh, Abdul Hadi
Soorni, Aboozar
Shoorooei, Marie
Torkzadeh Mahani, Masoud
Maali Amiri, Reza
Allahyari, Hossein
Mohammadi, Rahmat
author_sort Hoseinzadeh, Abdul Hadi
collection PubMed
description Common bean (Phaseolus vulgaris L.) is a major source of proteins and one of the most important edible foods for more than three hundred million people in the world. The common bean plants are frequently attacked by spider mite (Tetranychus urticae Koch), leading to a significant decrease in plant growth and economic performance. The use of resistant cultivars and the identification of the genes involved in plant-mite resistance are practical solutions to this problem. Hence, a comprehensive study of the molecular interactions between resistant and susceptible common bean cultivars and spider mite can shed light into the understanding of mechanisms and biological pathways of resistance. In this study, one resistant (Naz) and one susceptible (Akhtar) cultivars were selected for a transcriptome comparison at different time points (0, 1 and 5 days) after spider mite feeding. The comparison of cultivars in different time points revealed several key genes, which showed a change increase in transcript abundance via spider mite infestation. These included genes involved in flavonoid biosynthesis process; a conserved MYB-bHLH-WD40 (MBW) regulatory complex; transcription factors (TFs) TT2, TT8, TCP, Cys2/His2-type and C2H2-type zinc finger proteins; the ethylene response factors (ERFs) ERF1 and ERF9; genes related to metabolism of auxin and jasmonic acid (JA); pathogenesis-related (PR) proteins and heat shock proteins.
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spelling pubmed-69998992020-02-18 Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars Hoseinzadeh, Abdul Hadi Soorni, Aboozar Shoorooei, Marie Torkzadeh Mahani, Masoud Maali Amiri, Reza Allahyari, Hossein Mohammadi, Rahmat PLoS One Research Article Common bean (Phaseolus vulgaris L.) is a major source of proteins and one of the most important edible foods for more than three hundred million people in the world. The common bean plants are frequently attacked by spider mite (Tetranychus urticae Koch), leading to a significant decrease in plant growth and economic performance. The use of resistant cultivars and the identification of the genes involved in plant-mite resistance are practical solutions to this problem. Hence, a comprehensive study of the molecular interactions between resistant and susceptible common bean cultivars and spider mite can shed light into the understanding of mechanisms and biological pathways of resistance. In this study, one resistant (Naz) and one susceptible (Akhtar) cultivars were selected for a transcriptome comparison at different time points (0, 1 and 5 days) after spider mite feeding. The comparison of cultivars in different time points revealed several key genes, which showed a change increase in transcript abundance via spider mite infestation. These included genes involved in flavonoid biosynthesis process; a conserved MYB-bHLH-WD40 (MBW) regulatory complex; transcription factors (TFs) TT2, TT8, TCP, Cys2/His2-type and C2H2-type zinc finger proteins; the ethylene response factors (ERFs) ERF1 and ERF9; genes related to metabolism of auxin and jasmonic acid (JA); pathogenesis-related (PR) proteins and heat shock proteins. Public Library of Science 2020-02-04 /pmc/articles/PMC6999899/ /pubmed/32017794 http://dx.doi.org/10.1371/journal.pone.0228680 Text en © 2020 Hoseinzadeh et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hoseinzadeh, Abdul Hadi
Soorni, Aboozar
Shoorooei, Marie
Torkzadeh Mahani, Masoud
Maali Amiri, Reza
Allahyari, Hossein
Mohammadi, Rahmat
Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars
title Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars
title_full Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars
title_fullStr Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars
title_full_unstemmed Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars
title_short Comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars
title_sort comparative transcriptome provides molecular insight into defense-associated mechanisms against spider mite in resistant and susceptible common bean cultivars
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999899/
https://www.ncbi.nlm.nih.gov/pubmed/32017794
http://dx.doi.org/10.1371/journal.pone.0228680
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