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BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH

Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma. H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes. Th...

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Autores principales: Quintana-Hayashi, Macarena P., Rocha, Raquel, Padra, Médea, Thorell, Anders, Jin, Chunsheng, Karlsson, Niclas G., Roxo-Rosa, Mónica, Oleastro, Mónica, Lindén, Sara K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000205/
https://www.ncbi.nlm.nih.gov/pubmed/30298790
http://dx.doi.org/10.1080/21505594.2018.1532243
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author Quintana-Hayashi, Macarena P.
Rocha, Raquel
Padra, Médea
Thorell, Anders
Jin, Chunsheng
Karlsson, Niclas G.
Roxo-Rosa, Mónica
Oleastro, Mónica
Lindén, Sara K.
author_facet Quintana-Hayashi, Macarena P.
Rocha, Raquel
Padra, Médea
Thorell, Anders
Jin, Chunsheng
Karlsson, Niclas G.
Roxo-Rosa, Mónica
Oleastro, Mónica
Lindén, Sara K.
author_sort Quintana-Hayashi, Macarena P.
collection PubMed
description Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma. H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes. The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins. Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Le(b)), Sialyl-Lewis x (SLe(x)) or LacdiNAc. In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Le(b) was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Le(b) at acidic and neutral pH, and to SLe(x) and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity.
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spelling pubmed-70002052020-02-19 BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH Quintana-Hayashi, Macarena P. Rocha, Raquel Padra, Médea Thorell, Anders Jin, Chunsheng Karlsson, Niclas G. Roxo-Rosa, Mónica Oleastro, Mónica Lindén, Sara K. Virulence Research Paper Helicobacter pylori infection can result in non-ulcer dyspepsia (NUD), peptic ulcer disease (PUD), adenocarcinoma, and gastric lymphoma. H. pylori reside within the gastric mucus layer, mainly composed of mucins carrying an array of glycan structures that can serve as bacterial adhesion epitopes. The aim of the present study was to characterize the binding ability, adhesion modes, and growth of H. pylori strains from pediatric patients with NUD and PUD to gastric mucins. Our results showed an increased adhesion capacity of pediatric PUD H. pylori strains to human and rhesus monkey gastric mucins compared to the NUD strains both at neutral and acidic pH, regardless if the mucins were positive for Lewis b (Le(b)), Sialyl-Lewis x (SLe(x)) or LacdiNAc. In addition to babA positive strains being more common among PUD associated strains, H. pylori babA positive strains bound more avidly to gastric mucins than NUD babA positive strains at acidic pH. Binding to Le(b) was higher among babA positive PUD H. pylori strains compared to NUD strains at neutral, but not acidic, pH. PUD derived babA-knockout mutants had attenuated binding to mucins and Le(b) at acidic and neutral pH, and to SLe(x) and DNA at acidic pH. The results highlight the role of BabA-mediated adherence of pediatric ulcerogenic H. pylori strains, and points to a role for BabA in adhesion to charged structures at acidic pH, separate from its specific blood group binding activity. Taylor & Francis 2018-11-05 /pmc/articles/PMC7000205/ /pubmed/30298790 http://dx.doi.org/10.1080/21505594.2018.1532243 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Quintana-Hayashi, Macarena P.
Rocha, Raquel
Padra, Médea
Thorell, Anders
Jin, Chunsheng
Karlsson, Niclas G.
Roxo-Rosa, Mónica
Oleastro, Mónica
Lindén, Sara K.
BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_full BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_fullStr BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_full_unstemmed BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_short BabA-mediated adherence of pediatric ulcerogenic H. pylori strains to gastric mucins at neutral and acidic pH
title_sort baba-mediated adherence of pediatric ulcerogenic h. pylori strains to gastric mucins at neutral and acidic ph
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000205/
https://www.ncbi.nlm.nih.gov/pubmed/30298790
http://dx.doi.org/10.1080/21505594.2018.1532243
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