Cargando…

A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea

Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate...

Descripción completa

Detalles Bibliográficos
Autores principales: Vu, Diem-Lan, Sabrià, Aurora, Aregall, Nuria, Michl, Kristina, Sabrià, Jaume, Rodriguez Garrido, Virginia, Goterris, Lidia, Bosch, Albert, Pintó, Rosa Maria, Guix, Susana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000717/
https://www.ncbi.nlm.nih.gov/pubmed/32020041
http://dx.doi.org/10.1038/s41598-020-58691-3
_version_ 1783494093126500352
author Vu, Diem-Lan
Sabrià, Aurora
Aregall, Nuria
Michl, Kristina
Sabrià, Jaume
Rodriguez Garrido, Virginia
Goterris, Lidia
Bosch, Albert
Pintó, Rosa Maria
Guix, Susana
author_facet Vu, Diem-Lan
Sabrià, Aurora
Aregall, Nuria
Michl, Kristina
Sabrià, Jaume
Rodriguez Garrido, Virginia
Goterris, Lidia
Bosch, Albert
Pintó, Rosa Maria
Guix, Susana
author_sort Vu, Diem-Lan
collection PubMed
description Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate novel HAstV in a gastroenteritis case-control study including a pediatric population in Spain over a one-year period. We included stool samples from patients with gastroenteritis and negative results for viruses screened by routine diagnostics, and stool samples of control subjects who sought for a routine medical consultation. All samples were screened by real-time RT-PCR assays for novel HAstV. An additional screening for rotavirus, norovirus GI, GII, sapovirus, classic HAstV and adenovirus was also performed for the control group. Overall, 23/363 stool samples from case patients (6.3%) and 8/199 stool samples from control patients (4%) were positive for ≥1 novel HAstV. MLB1 was predominant (64.5% of positives). Seasonality was observed for the case group (p = 0.015), but not the control group (p = 0.95). No difference was observed in the prevalence of novel HAstV between the case and control groups (OR 1.78, 95% CI 0.68–5.45; p = 0.30). Nevertheless, MLB genome copy numbers/ml of fecal suspension was significantly higher in the control group than in the case group (p = 0.008). In our study, we identified a lack of association between novel HAstV and gastroenteritis in the studied population, which could indicate a potential role of reservoir for children, especially given the higher viral load observed in the asymptomatic group for some of them.
format Online
Article
Text
id pubmed-7000717
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-70007172020-02-11 A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea Vu, Diem-Lan Sabrià, Aurora Aregall, Nuria Michl, Kristina Sabrià, Jaume Rodriguez Garrido, Virginia Goterris, Lidia Bosch, Albert Pintó, Rosa Maria Guix, Susana Sci Rep Article Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate novel HAstV in a gastroenteritis case-control study including a pediatric population in Spain over a one-year period. We included stool samples from patients with gastroenteritis and negative results for viruses screened by routine diagnostics, and stool samples of control subjects who sought for a routine medical consultation. All samples were screened by real-time RT-PCR assays for novel HAstV. An additional screening for rotavirus, norovirus GI, GII, sapovirus, classic HAstV and adenovirus was also performed for the control group. Overall, 23/363 stool samples from case patients (6.3%) and 8/199 stool samples from control patients (4%) were positive for ≥1 novel HAstV. MLB1 was predominant (64.5% of positives). Seasonality was observed for the case group (p = 0.015), but not the control group (p = 0.95). No difference was observed in the prevalence of novel HAstV between the case and control groups (OR 1.78, 95% CI 0.68–5.45; p = 0.30). Nevertheless, MLB genome copy numbers/ml of fecal suspension was significantly higher in the control group than in the case group (p = 0.008). In our study, we identified a lack of association between novel HAstV and gastroenteritis in the studied population, which could indicate a potential role of reservoir for children, especially given the higher viral load observed in the asymptomatic group for some of them. Nature Publishing Group UK 2020-02-04 /pmc/articles/PMC7000717/ /pubmed/32020041 http://dx.doi.org/10.1038/s41598-020-58691-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Vu, Diem-Lan
Sabrià, Aurora
Aregall, Nuria
Michl, Kristina
Sabrià, Jaume
Rodriguez Garrido, Virginia
Goterris, Lidia
Bosch, Albert
Pintó, Rosa Maria
Guix, Susana
A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
title A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
title_full A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
title_fullStr A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
title_full_unstemmed A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
title_short A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
title_sort spanish case-control study in <5 year-old children reveals the lack of association between mlb and va astrovirus and diarrhea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000717/
https://www.ncbi.nlm.nih.gov/pubmed/32020041
http://dx.doi.org/10.1038/s41598-020-58691-3
work_keys_str_mv AT vudiemlan aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT sabriaaurora aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT aregallnuria aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT michlkristina aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT sabriajaume aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT rodriguezgarridovirginia aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT goterrislidia aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT boschalbert aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT pintorosamaria aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT guixsusana aspanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT vudiemlan spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT sabriaaurora spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT aregallnuria spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT michlkristina spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT sabriajaume spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT rodriguezgarridovirginia spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT goterrislidia spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT boschalbert spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT pintorosamaria spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea
AT guixsusana spanishcasecontrolstudyin5yearoldchildrenrevealsthelackofassociationbetweenmlbandvaastrovirusanddiarrhea