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A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000717/ https://www.ncbi.nlm.nih.gov/pubmed/32020041 http://dx.doi.org/10.1038/s41598-020-58691-3 |
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author | Vu, Diem-Lan Sabrià, Aurora Aregall, Nuria Michl, Kristina Sabrià, Jaume Rodriguez Garrido, Virginia Goterris, Lidia Bosch, Albert Pintó, Rosa Maria Guix, Susana |
author_facet | Vu, Diem-Lan Sabrià, Aurora Aregall, Nuria Michl, Kristina Sabrià, Jaume Rodriguez Garrido, Virginia Goterris, Lidia Bosch, Albert Pintó, Rosa Maria Guix, Susana |
author_sort | Vu, Diem-Lan |
collection | PubMed |
description | Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate novel HAstV in a gastroenteritis case-control study including a pediatric population in Spain over a one-year period. We included stool samples from patients with gastroenteritis and negative results for viruses screened by routine diagnostics, and stool samples of control subjects who sought for a routine medical consultation. All samples were screened by real-time RT-PCR assays for novel HAstV. An additional screening for rotavirus, norovirus GI, GII, sapovirus, classic HAstV and adenovirus was also performed for the control group. Overall, 23/363 stool samples from case patients (6.3%) and 8/199 stool samples from control patients (4%) were positive for ≥1 novel HAstV. MLB1 was predominant (64.5% of positives). Seasonality was observed for the case group (p = 0.015), but not the control group (p = 0.95). No difference was observed in the prevalence of novel HAstV between the case and control groups (OR 1.78, 95% CI 0.68–5.45; p = 0.30). Nevertheless, MLB genome copy numbers/ml of fecal suspension was significantly higher in the control group than in the case group (p = 0.008). In our study, we identified a lack of association between novel HAstV and gastroenteritis in the studied population, which could indicate a potential role of reservoir for children, especially given the higher viral load observed in the asymptomatic group for some of them. |
format | Online Article Text |
id | pubmed-7000717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70007172020-02-11 A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea Vu, Diem-Lan Sabrià, Aurora Aregall, Nuria Michl, Kristina Sabrià, Jaume Rodriguez Garrido, Virginia Goterris, Lidia Bosch, Albert Pintó, Rosa Maria Guix, Susana Sci Rep Article Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate novel HAstV in a gastroenteritis case-control study including a pediatric population in Spain over a one-year period. We included stool samples from patients with gastroenteritis and negative results for viruses screened by routine diagnostics, and stool samples of control subjects who sought for a routine medical consultation. All samples were screened by real-time RT-PCR assays for novel HAstV. An additional screening for rotavirus, norovirus GI, GII, sapovirus, classic HAstV and adenovirus was also performed for the control group. Overall, 23/363 stool samples from case patients (6.3%) and 8/199 stool samples from control patients (4%) were positive for ≥1 novel HAstV. MLB1 was predominant (64.5% of positives). Seasonality was observed for the case group (p = 0.015), but not the control group (p = 0.95). No difference was observed in the prevalence of novel HAstV between the case and control groups (OR 1.78, 95% CI 0.68–5.45; p = 0.30). Nevertheless, MLB genome copy numbers/ml of fecal suspension was significantly higher in the control group than in the case group (p = 0.008). In our study, we identified a lack of association between novel HAstV and gastroenteritis in the studied population, which could indicate a potential role of reservoir for children, especially given the higher viral load observed in the asymptomatic group for some of them. Nature Publishing Group UK 2020-02-04 /pmc/articles/PMC7000717/ /pubmed/32020041 http://dx.doi.org/10.1038/s41598-020-58691-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Vu, Diem-Lan Sabrià, Aurora Aregall, Nuria Michl, Kristina Sabrià, Jaume Rodriguez Garrido, Virginia Goterris, Lidia Bosch, Albert Pintó, Rosa Maria Guix, Susana A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea |
title | A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea |
title_full | A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea |
title_fullStr | A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea |
title_full_unstemmed | A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea |
title_short | A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea |
title_sort | spanish case-control study in <5 year-old children reveals the lack of association between mlb and va astrovirus and diarrhea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000717/ https://www.ncbi.nlm.nih.gov/pubmed/32020041 http://dx.doi.org/10.1038/s41598-020-58691-3 |
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