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Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix

Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. Th...

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Autores principales: Stachnik, Karolina, Warmer, Martin, Mohacsi, Istvan, Hennicke, Vincent, Fischer, Pontus, Meyer, Jan, Spitzbart, Tobias, Barthelmess, Miriam, Eich, Jacqueline, David, Christian, Feldmann, Claus, Busse, Björn, Jähn, Katharina, Schaible, Ulrich E., Meents, Alke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000813/
https://www.ncbi.nlm.nih.gov/pubmed/32019946
http://dx.doi.org/10.1038/s41598-020-58318-7
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author Stachnik, Karolina
Warmer, Martin
Mohacsi, Istvan
Hennicke, Vincent
Fischer, Pontus
Meyer, Jan
Spitzbart, Tobias
Barthelmess, Miriam
Eich, Jacqueline
David, Christian
Feldmann, Claus
Busse, Björn
Jähn, Katharina
Schaible, Ulrich E.
Meents, Alke
author_facet Stachnik, Karolina
Warmer, Martin
Mohacsi, Istvan
Hennicke, Vincent
Fischer, Pontus
Meyer, Jan
Spitzbart, Tobias
Barthelmess, Miriam
Eich, Jacqueline
David, Christian
Feldmann, Claus
Busse, Björn
Jähn, Katharina
Schaible, Ulrich E.
Meents, Alke
author_sort Stachnik, Karolina
collection PubMed
description Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. They enable a robust correlation of elemental distributions with respect to the cellular morphology. Here we extend the applicability of the two modalities to higher X-ray excitation energies, permitting iron mapping. Using a long-range scanning setup, we applied the method to two vital biomedical cases. We quantified the iron distributions in a population of macrophages treated with Mycobacterium-tuberculosis-targeting iron-oxide nanocontainers. Our work allowed to visualize the internalization of the nanocontainer agglomerates in the cytosol. From the iron areal mass maps, we obtained a distribution of antibiotic load per agglomerate and an average areal concentration of nanocontainers in the agglomerates. In the second application we mapped the calcium content in a human bone matrix in close proximity to osteocyte lacunae (perilacunar matrix). A concurrently acquired ptychographic image was used to remove the mass-thickness effect from the raw calcium map. The resulting ptychography-enhanced calcium distribution allowed then to observe a locally lower degree of mineralization of the perilacunar matrix.
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spelling pubmed-70008132020-02-11 Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix Stachnik, Karolina Warmer, Martin Mohacsi, Istvan Hennicke, Vincent Fischer, Pontus Meyer, Jan Spitzbart, Tobias Barthelmess, Miriam Eich, Jacqueline David, Christian Feldmann, Claus Busse, Björn Jähn, Katharina Schaible, Ulrich E. Meents, Alke Sci Rep Article Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. They enable a robust correlation of elemental distributions with respect to the cellular morphology. Here we extend the applicability of the two modalities to higher X-ray excitation energies, permitting iron mapping. Using a long-range scanning setup, we applied the method to two vital biomedical cases. We quantified the iron distributions in a population of macrophages treated with Mycobacterium-tuberculosis-targeting iron-oxide nanocontainers. Our work allowed to visualize the internalization of the nanocontainer agglomerates in the cytosol. From the iron areal mass maps, we obtained a distribution of antibiotic load per agglomerate and an average areal concentration of nanocontainers in the agglomerates. In the second application we mapped the calcium content in a human bone matrix in close proximity to osteocyte lacunae (perilacunar matrix). A concurrently acquired ptychographic image was used to remove the mass-thickness effect from the raw calcium map. The resulting ptychography-enhanced calcium distribution allowed then to observe a locally lower degree of mineralization of the perilacunar matrix. Nature Publishing Group UK 2020-02-04 /pmc/articles/PMC7000813/ /pubmed/32019946 http://dx.doi.org/10.1038/s41598-020-58318-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Stachnik, Karolina
Warmer, Martin
Mohacsi, Istvan
Hennicke, Vincent
Fischer, Pontus
Meyer, Jan
Spitzbart, Tobias
Barthelmess, Miriam
Eich, Jacqueline
David, Christian
Feldmann, Claus
Busse, Björn
Jähn, Katharina
Schaible, Ulrich E.
Meents, Alke
Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
title Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
title_full Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
title_fullStr Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
title_full_unstemmed Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
title_short Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
title_sort multimodal x-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000813/
https://www.ncbi.nlm.nih.gov/pubmed/32019946
http://dx.doi.org/10.1038/s41598-020-58318-7
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