Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip

Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infect...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jung-Min, Lim, Su-Jin, Kim, SoMin, Kim, MoonJung, Kim, ByoungHee, Tai, Truong A, Kim, Seonmi, Yoon, ByoungSu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000893/
https://www.ncbi.nlm.nih.gov/pubmed/31940683
http://dx.doi.org/10.4142/jvs.2020.21.e4
Descripción
Sumario:Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.

Ejemplares similares