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Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis

OBJECTIVE: Andrographis paniculata, widely used as an antidiabetic in Indonesian traditional medicines (jamu), contains chemical compounds whose concentration is related to its therapeutic effects. The concentration of solvents used for extraction will also affect the number of compounds extracted....

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Autores principales: Rafi, M., Devi, A. F., Syafitri, U. D., Heryanto, R., Suparto, I. H., Amran, M. B., Rohman, A., Prajogo, B., Lim, L. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001318/
https://www.ncbi.nlm.nih.gov/pubmed/32019574
http://dx.doi.org/10.1186/s13104-020-4920-x
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author Rafi, M.
Devi, A. F.
Syafitri, U. D.
Heryanto, R.
Suparto, I. H.
Amran, M. B.
Rohman, A.
Prajogo, B.
Lim, L. W.
author_facet Rafi, M.
Devi, A. F.
Syafitri, U. D.
Heryanto, R.
Suparto, I. H.
Amran, M. B.
Rohman, A.
Prajogo, B.
Lim, L. W.
author_sort Rafi, M.
collection PubMed
description OBJECTIVE: Andrographis paniculata, widely used as an antidiabetic in Indonesian traditional medicines (jamu), contains chemical compounds whose concentration is related to its therapeutic effects. The concentration of solvents used for extraction will also affect the number of compounds extracted. Therefore, a quality control method is needed to ensure consistency in quantifying these compounds in A. paniculata to improve its therapeutic application. High-performance liquid chromatography fingerprint analysis combined with chemometrics was used to evaluate extracts from different solvent extraction treatments. The content of andrographolide, the main bioactive compound in A. paniculata, and the level of α-glucosidase inhibition activity, an indicator of its antidiabetic activity, were also determined. RESULTS: Fingerprint chromatograms of A. paniculata extracts from different treatments exhibited a similar pattern with several peaks in common, only differing in area and intensity value. The A. paniculata extracts were classified using HPLC fingerprint and principal component analysis to allow grouping according to their respective solvent extraction treatments. The highest andrographolide content and α-glucosidase inhibition activity occurred in the 50% ethanol extract and the lowest in the water extract. HPLC fingerprint analysis could be used for identifying A. paniculata extracts based on solvent extraction, thus improving quality control for their therapeutic application.
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spelling pubmed-70013182020-02-10 Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis Rafi, M. Devi, A. F. Syafitri, U. D. Heryanto, R. Suparto, I. H. Amran, M. B. Rohman, A. Prajogo, B. Lim, L. W. BMC Res Notes Research Note OBJECTIVE: Andrographis paniculata, widely used as an antidiabetic in Indonesian traditional medicines (jamu), contains chemical compounds whose concentration is related to its therapeutic effects. The concentration of solvents used for extraction will also affect the number of compounds extracted. Therefore, a quality control method is needed to ensure consistency in quantifying these compounds in A. paniculata to improve its therapeutic application. High-performance liquid chromatography fingerprint analysis combined with chemometrics was used to evaluate extracts from different solvent extraction treatments. The content of andrographolide, the main bioactive compound in A. paniculata, and the level of α-glucosidase inhibition activity, an indicator of its antidiabetic activity, were also determined. RESULTS: Fingerprint chromatograms of A. paniculata extracts from different treatments exhibited a similar pattern with several peaks in common, only differing in area and intensity value. The A. paniculata extracts were classified using HPLC fingerprint and principal component analysis to allow grouping according to their respective solvent extraction treatments. The highest andrographolide content and α-glucosidase inhibition activity occurred in the 50% ethanol extract and the lowest in the water extract. HPLC fingerprint analysis could be used for identifying A. paniculata extracts based on solvent extraction, thus improving quality control for their therapeutic application. BioMed Central 2020-02-04 /pmc/articles/PMC7001318/ /pubmed/32019574 http://dx.doi.org/10.1186/s13104-020-4920-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Rafi, M.
Devi, A. F.
Syafitri, U. D.
Heryanto, R.
Suparto, I. H.
Amran, M. B.
Rohman, A.
Prajogo, B.
Lim, L. W.
Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis
title Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis
title_full Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis
title_fullStr Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis
title_full_unstemmed Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis
title_short Classification of Andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis
title_sort classification of andrographis paniculata extracts by solvent extraction using hplc fingerprint and chemometric analysis
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001318/
https://www.ncbi.nlm.nih.gov/pubmed/32019574
http://dx.doi.org/10.1186/s13104-020-4920-x
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