Cargando…

Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model

BACKGROUND: Regeneration of bone defects remains a challenge for maxillofacial surgeons. The objective of this study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination with together in artificially created mandibular bone defe...

Descripción completa

Detalles Bibliográficos
Autores principales: Sargolzaei-Aval, Fereydoon, Saberi, Eshagh Ali, Arab, Mohammad Reza, Sargolzaei, Narjes, Zare, Esmaeel, Shahraki, Heshmatollah, Sanchooli, Tayebeh, Sargolzaeiaval, Forough, Arab, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001562/
https://www.ncbi.nlm.nih.gov/pubmed/32055288
_version_ 1783494267123007488
author Sargolzaei-Aval, Fereydoon
Saberi, Eshagh Ali
Arab, Mohammad Reza
Sargolzaei, Narjes
Zare, Esmaeel
Shahraki, Heshmatollah
Sanchooli, Tayebeh
Sargolzaeiaval, Forough
Arab, Maryam
author_facet Sargolzaei-Aval, Fereydoon
Saberi, Eshagh Ali
Arab, Mohammad Reza
Sargolzaei, Narjes
Zare, Esmaeel
Shahraki, Heshmatollah
Sanchooli, Tayebeh
Sargolzaeiaval, Forough
Arab, Maryam
author_sort Sargolzaei-Aval, Fereydoon
collection PubMed
description BACKGROUND: Regeneration of bone defects remains a challenge for maxillofacial surgeons. The objective of this study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination with together in artificially created mandibular bone defects. MATERIALS AND METHODS: In this experimental study Forty-eight male Sprague–Dawley rats (6–8 weeks old) were randomly divided into four groups. Defects were created in the mandible of rats and filled with 10 mg of OCP, BMG, or a combination of both (1/4 ratio). Defects were left unfilled in the control group. To assess bone regeneration and determine the amount of the newly formed bone, specimens were harvested at 7, 14, 21, and 56 days postimplantation. The specimens were processed routinely and studied histologically and histomorphometrically using the light microscope and eyepiece graticule. The amount of newly formed bone was quantitatively measured using histomorphometric methods. Histomorphometric data were analyzed using SPSS software. Mean, standard deviation, mode, and medians were calculated. Tukey HSD test was used to compare the means in all groups. P < 0.05 was considered as statistically significant (i.e., 5% significant level). RESULTS: In the experimental groups, the new bone formation was initiated from the margin of defects during the 7–14 days after implantation. By the end of study, the amount of newly formed bone increased and relatively matured, and almost all of the implanted materials were absorbed. In the control group, slight amount of new bone had been formed at the defect margins (next to the host bone) on day 56. The histomorphometric analysis revealed statistically significant differences in the amount of newly formed bone between the experimental and the control groups (P < 0.001). CONCLUSION: Combination of OCP/BMG may serve as an optimal biomaterial for the treatment of mandibular bone defects.
format Online
Article
Text
id pubmed-7001562
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-70015622020-02-13 Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model Sargolzaei-Aval, Fereydoon Saberi, Eshagh Ali Arab, Mohammad Reza Sargolzaei, Narjes Zare, Esmaeel Shahraki, Heshmatollah Sanchooli, Tayebeh Sargolzaeiaval, Forough Arab, Maryam Dent Res J (Isfahan) Original Article BACKGROUND: Regeneration of bone defects remains a challenge for maxillofacial surgeons. The objective of this study was to assess the osteogenic potential of octacalcium phosphate (OCP) and bone matrix gelatin (BMG) alone and in combination with together in artificially created mandibular bone defects. MATERIALS AND METHODS: In this experimental study Forty-eight male Sprague–Dawley rats (6–8 weeks old) were randomly divided into four groups. Defects were created in the mandible of rats and filled with 10 mg of OCP, BMG, or a combination of both (1/4 ratio). Defects were left unfilled in the control group. To assess bone regeneration and determine the amount of the newly formed bone, specimens were harvested at 7, 14, 21, and 56 days postimplantation. The specimens were processed routinely and studied histologically and histomorphometrically using the light microscope and eyepiece graticule. The amount of newly formed bone was quantitatively measured using histomorphometric methods. Histomorphometric data were analyzed using SPSS software. Mean, standard deviation, mode, and medians were calculated. Tukey HSD test was used to compare the means in all groups. P < 0.05 was considered as statistically significant (i.e., 5% significant level). RESULTS: In the experimental groups, the new bone formation was initiated from the margin of defects during the 7–14 days after implantation. By the end of study, the amount of newly formed bone increased and relatively matured, and almost all of the implanted materials were absorbed. In the control group, slight amount of new bone had been formed at the defect margins (next to the host bone) on day 56. The histomorphometric analysis revealed statistically significant differences in the amount of newly formed bone between the experimental and the control groups (P < 0.001). CONCLUSION: Combination of OCP/BMG may serve as an optimal biomaterial for the treatment of mandibular bone defects. Wolters Kluwer - Medknow 2020-01-21 /pmc/articles/PMC7001562/ /pubmed/32055288 Text en Copyright: © 2020 Dental Research Journal http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sargolzaei-Aval, Fereydoon
Saberi, Eshagh Ali
Arab, Mohammad Reza
Sargolzaei, Narjes
Zare, Esmaeel
Shahraki, Heshmatollah
Sanchooli, Tayebeh
Sargolzaeiaval, Forough
Arab, Maryam
Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model
title Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model
title_full Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model
title_fullStr Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model
title_full_unstemmed Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model
title_short Reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model
title_sort reconstruction of mandibular defects using synthetic octacalcium phosphate combined with bone matrix gelatin in rat model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001562/
https://www.ncbi.nlm.nih.gov/pubmed/32055288
work_keys_str_mv AT sargolzaeiavalfereydoon reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT saberieshaghali reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT arabmohammadreza reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT sargolzaeinarjes reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT zareesmaeel reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT shahrakiheshmatollah reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT sanchoolitayebeh reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT sargolzaeiavalforough reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel
AT arabmaryam reconstructionofmandibulardefectsusingsyntheticoctacalciumphosphatecombinedwithbonematrixgelatininratmodel