High‐Throughput Imaging: Fast, 3D Isotropic Imaging of Whole Mouse Brain Using Multiangle‐Resolved Subvoxel SPIM (Adv. Sci. 3/2020)

In article number https://doi.org/10.1002/advs.201901891, Dan Zhu, Peng Fei, and co‐workers develop an imaging technique, multiangle‐resolved subvoxel selective plane illumination microscopy (termed Mars‐SPIM), that can readily obtain the 3D digital atlas of a cleared whole mouse brain with an isotr...

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Detalles Bibliográficos
Autores principales: Nie, Jun, Liu, Sa, Yu, Tingting, Li, Yusha, Ping, Junyu, Wan, Peng, Zhao, Fang, Huang, Yujie, Mei, Wei, Zeng, Shaoqun, Zhu, Dan, Fei, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Frontispiece
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001636/
http://dx.doi.org/10.1002/advs.202070015
Descripción
Sumario:In article number https://doi.org/10.1002/advs.201901891, Dan Zhu, Peng Fei, and co‐workers develop an imaging technique, multiangle‐resolved subvoxel selective plane illumination microscopy (termed Mars‐SPIM), that can readily obtain the 3D digital atlas of a cleared whole mouse brain with an isotropic single‐cell resolution, in a short acquisition time of several minutes. It provides an efficient way to implement system‐level cellular analysis, such as the mapping of different neuron populations and tracing of long‐distance neural projections over the entire brain. [Image: see text]

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