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Three-Dimensional Visualization of APEX2-Tagged Erg11 in Saccharomyces cerevisiae Using Focused Ion Beam Scanning Electron Microscopy
The determination of the exact location of a protein in the cell is essential to the understanding of biological processes. Here, we report for the first time the visualization of a protein of interest in Saccharomyces cerevisiae using focused ion beam scanning electron microscopy (FIB-SEM). As a pr...
Autores principales: | Kerstens, Winnie, Kremer, Anneke, Holtappels, Michelle, Borghgraef, Peter, Lippens, Saskia, Van Dijck, Patrick |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002314/ https://www.ncbi.nlm.nih.gov/pubmed/32024705 http://dx.doi.org/10.1128/mSphere.00981-19 |
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