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Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice

Chronic kidney disease (CKD) progresses to end-stage renal failure via renal tubulointerstitial fibrosis. Malnutrition, inflammation, and arteriosclerosis interact to exacerbate the poor prognosis of CKD, and their effective management is thus essential. The traditional Japanese medicine Rikkunshito...

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Autores principales: Wakui, Hiromichi, Yamaji, Takahiro, Azushima, Kengo, Uneda, Kazushi, Haruhara, Kotaro, Nakamura, Akiko, Ohki, Kohji, Kinguchi, Sho, Kobayashi, Ryu, Urate, Shingo, Suzuki, Toru, Kamimura, Daisuke, Minegishi, Shintaro, Ishigami, Tomoaki, Kanaoka, Tomohiko, Matsuo, Kohei, Miyazaki, Tomoyuki, Fujikawa, Tetsuya, Yamashita, Akio, Tamura, Kouichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002622/
https://www.ncbi.nlm.nih.gov/pubmed/32024850
http://dx.doi.org/10.1038/s41598-020-58214-0
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author Wakui, Hiromichi
Yamaji, Takahiro
Azushima, Kengo
Uneda, Kazushi
Haruhara, Kotaro
Nakamura, Akiko
Ohki, Kohji
Kinguchi, Sho
Kobayashi, Ryu
Urate, Shingo
Suzuki, Toru
Kamimura, Daisuke
Minegishi, Shintaro
Ishigami, Tomoaki
Kanaoka, Tomohiko
Matsuo, Kohei
Miyazaki, Tomoyuki
Fujikawa, Tetsuya
Yamashita, Akio
Tamura, Kouichi
author_facet Wakui, Hiromichi
Yamaji, Takahiro
Azushima, Kengo
Uneda, Kazushi
Haruhara, Kotaro
Nakamura, Akiko
Ohki, Kohji
Kinguchi, Sho
Kobayashi, Ryu
Urate, Shingo
Suzuki, Toru
Kamimura, Daisuke
Minegishi, Shintaro
Ishigami, Tomoaki
Kanaoka, Tomohiko
Matsuo, Kohei
Miyazaki, Tomoyuki
Fujikawa, Tetsuya
Yamashita, Akio
Tamura, Kouichi
author_sort Wakui, Hiromichi
collection PubMed
description Chronic kidney disease (CKD) progresses to end-stage renal failure via renal tubulointerstitial fibrosis. Malnutrition, inflammation, and arteriosclerosis interact to exacerbate the poor prognosis of CKD, and their effective management is thus essential. The traditional Japanese medicine Rikkunshito (RKT) exerts appetite-stimulating effects via ghrelin, which attenuates inflammation and fibrosis. We evaluated the therapeutic effect of RKT in unilateral ureter obstruction (UUO)-induced renal fibrosis/inflammation and body weight loss in mice. UUO and sham-operated mice were fed a standard diet or diet containing 3.0% RKT. Renal fibrosis was investigated by histopathology and macrophage infiltration was determined by immunohistochemistry. Expression levels of genes associated with fibrosis, inflammation, ghrelin, and mitochondrial function were determined by quantitative reverse transcription-polymerase chain reaction and western blot analyses. RKT treatment partially prevented UUO-induced weight loss but failed to attenuate renal fibrosis and inflammation. Renal expression of sirtuin 1, a ghrelin-downstream signalling molecule, and gene expression of peroxisome proliferator-activated receptor-γ coactivator 1α and Bcl-2/adenovirus E1B interacting protein 3 were unaffected by RKT. These results indicate that RKT inhibits weight loss but does not improve renal fibrosis or inflammation in a rapidly progressive renal fibrosis mouse model. RKT may have a protective effect on weight loss associated with CKD.
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spelling pubmed-70026222020-02-14 Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice Wakui, Hiromichi Yamaji, Takahiro Azushima, Kengo Uneda, Kazushi Haruhara, Kotaro Nakamura, Akiko Ohki, Kohji Kinguchi, Sho Kobayashi, Ryu Urate, Shingo Suzuki, Toru Kamimura, Daisuke Minegishi, Shintaro Ishigami, Tomoaki Kanaoka, Tomohiko Matsuo, Kohei Miyazaki, Tomoyuki Fujikawa, Tetsuya Yamashita, Akio Tamura, Kouichi Sci Rep Article Chronic kidney disease (CKD) progresses to end-stage renal failure via renal tubulointerstitial fibrosis. Malnutrition, inflammation, and arteriosclerosis interact to exacerbate the poor prognosis of CKD, and their effective management is thus essential. The traditional Japanese medicine Rikkunshito (RKT) exerts appetite-stimulating effects via ghrelin, which attenuates inflammation and fibrosis. We evaluated the therapeutic effect of RKT in unilateral ureter obstruction (UUO)-induced renal fibrosis/inflammation and body weight loss in mice. UUO and sham-operated mice were fed a standard diet or diet containing 3.0% RKT. Renal fibrosis was investigated by histopathology and macrophage infiltration was determined by immunohistochemistry. Expression levels of genes associated with fibrosis, inflammation, ghrelin, and mitochondrial function were determined by quantitative reverse transcription-polymerase chain reaction and western blot analyses. RKT treatment partially prevented UUO-induced weight loss but failed to attenuate renal fibrosis and inflammation. Renal expression of sirtuin 1, a ghrelin-downstream signalling molecule, and gene expression of peroxisome proliferator-activated receptor-γ coactivator 1α and Bcl-2/adenovirus E1B interacting protein 3 were unaffected by RKT. These results indicate that RKT inhibits weight loss but does not improve renal fibrosis or inflammation in a rapidly progressive renal fibrosis mouse model. RKT may have a protective effect on weight loss associated with CKD. Nature Publishing Group UK 2020-02-05 /pmc/articles/PMC7002622/ /pubmed/32024850 http://dx.doi.org/10.1038/s41598-020-58214-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wakui, Hiromichi
Yamaji, Takahiro
Azushima, Kengo
Uneda, Kazushi
Haruhara, Kotaro
Nakamura, Akiko
Ohki, Kohji
Kinguchi, Sho
Kobayashi, Ryu
Urate, Shingo
Suzuki, Toru
Kamimura, Daisuke
Minegishi, Shintaro
Ishigami, Tomoaki
Kanaoka, Tomohiko
Matsuo, Kohei
Miyazaki, Tomoyuki
Fujikawa, Tetsuya
Yamashita, Akio
Tamura, Kouichi
Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice
title Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice
title_full Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice
title_fullStr Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice
title_full_unstemmed Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice
title_short Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice
title_sort effects of rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002622/
https://www.ncbi.nlm.nih.gov/pubmed/32024850
http://dx.doi.org/10.1038/s41598-020-58214-0
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