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New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation
Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well-known, its exact molecular mechanisms remain widely undiscovered. Oocyte growth, differentiation and maturation are therefore...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002967/ https://www.ncbi.nlm.nih.gov/pubmed/32016446 http://dx.doi.org/10.3892/mmr.2020.10963 |
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author | Brązert, Maciej Kranc, Wiesława Nawrocki, Mariusz J. Sujka-Kordowska, Patrycja Konwerska, Aneta Jankowski, Maurycy Kocherova, Ievgeniia Celichowski, Piotr Jeseta, Michal Ożegowska, Katarzyna Antosik, Paweł Bukowska, Dorota Skowroński, Mariusz T. Bruska, Małgorzata Pawelczyk, Leszek Zabel, Maciej Piotrowska-Kempisty, Hanna Nowicki, Michał Kempisty, Bartosz |
author_facet | Brązert, Maciej Kranc, Wiesława Nawrocki, Mariusz J. Sujka-Kordowska, Patrycja Konwerska, Aneta Jankowski, Maurycy Kocherova, Ievgeniia Celichowski, Piotr Jeseta, Michal Ożegowska, Katarzyna Antosik, Paweł Bukowska, Dorota Skowroński, Mariusz T. Bruska, Małgorzata Pawelczyk, Leszek Zabel, Maciej Piotrowska-Kempisty, Hanna Nowicki, Michał Kempisty, Bartosz |
author_sort | Brązert, Maciej |
collection | PubMed |
description | Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well-known, its exact molecular mechanisms remain widely undiscovered. Oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature has indicated that the oocyte itself serves a regulatory role in the mechanisms underlying these processes. Hence, the present study performed expression microarrays to analyze the complete transcriptome of porcine oocytes during their in vitro maturation (IVM). Pig material was used for experimentation, as it possesses similarities to the reproductive processes and general genetic proximities of Sus scrofa to human. Oocytes, isolated from the ovaries of slaughtered animals were assessed via the Brilliant Cresyl Blue test and directed to IVM. A number of oocytes were left to be analyzed as the ‘before IVM’ group. Oocyte mRNA was isolated and used for microarray analysis, which was subsequently validated via RT-qPCR. The current study particularly focused on genes belonging to ‘positive regulation of transcription, DNA-dependent’, ‘positive regulation of gene expression’, ‘positive regulation of macromolecule metabolic process’ and ‘positive regulation of transcription from RNA polymerase II promoter’ ontologies. FOS, VEGFA, ESR1, AR, CCND2, EGR2, ENDRA, GJA1, INHBA, IHH, INSR, APP, WWTR1, SMARCA1, NFAT5, SMAD4, MAP3K1, EGR1, RORA, ECE1, NR5A1, KIT, IKZF2, MEF2C, SH3D19, MITF and PSMB4 were all determined to be significantly altered (fold change, >|2|; P<0.05) among these groups, with their downregulation being observed after IVM. Genes with the most altered expressions were analyzed and considered to be potential markers of maturation associated with transcription regulation and macromolecule metabolism process. |
format | Online Article Text |
id | pubmed-7002967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-70029672020-02-12 New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation Brązert, Maciej Kranc, Wiesława Nawrocki, Mariusz J. Sujka-Kordowska, Patrycja Konwerska, Aneta Jankowski, Maurycy Kocherova, Ievgeniia Celichowski, Piotr Jeseta, Michal Ożegowska, Katarzyna Antosik, Paweł Bukowska, Dorota Skowroński, Mariusz T. Bruska, Małgorzata Pawelczyk, Leszek Zabel, Maciej Piotrowska-Kempisty, Hanna Nowicki, Michał Kempisty, Bartosz Mol Med Rep Articles Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well-known, its exact molecular mechanisms remain widely undiscovered. Oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature has indicated that the oocyte itself serves a regulatory role in the mechanisms underlying these processes. Hence, the present study performed expression microarrays to analyze the complete transcriptome of porcine oocytes during their in vitro maturation (IVM). Pig material was used for experimentation, as it possesses similarities to the reproductive processes and general genetic proximities of Sus scrofa to human. Oocytes, isolated from the ovaries of slaughtered animals were assessed via the Brilliant Cresyl Blue test and directed to IVM. A number of oocytes were left to be analyzed as the ‘before IVM’ group. Oocyte mRNA was isolated and used for microarray analysis, which was subsequently validated via RT-qPCR. The current study particularly focused on genes belonging to ‘positive regulation of transcription, DNA-dependent’, ‘positive regulation of gene expression’, ‘positive regulation of macromolecule metabolic process’ and ‘positive regulation of transcription from RNA polymerase II promoter’ ontologies. FOS, VEGFA, ESR1, AR, CCND2, EGR2, ENDRA, GJA1, INHBA, IHH, INSR, APP, WWTR1, SMARCA1, NFAT5, SMAD4, MAP3K1, EGR1, RORA, ECE1, NR5A1, KIT, IKZF2, MEF2C, SH3D19, MITF and PSMB4 were all determined to be significantly altered (fold change, >|2|; P<0.05) among these groups, with their downregulation being observed after IVM. Genes with the most altered expressions were analyzed and considered to be potential markers of maturation associated with transcription regulation and macromolecule metabolism process. D.A. Spandidos 2020-03 2020-01-27 /pmc/articles/PMC7002967/ /pubmed/32016446 http://dx.doi.org/10.3892/mmr.2020.10963 Text en Copyright: © Brązert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Brązert, Maciej Kranc, Wiesława Nawrocki, Mariusz J. Sujka-Kordowska, Patrycja Konwerska, Aneta Jankowski, Maurycy Kocherova, Ievgeniia Celichowski, Piotr Jeseta, Michal Ożegowska, Katarzyna Antosik, Paweł Bukowska, Dorota Skowroński, Mariusz T. Bruska, Małgorzata Pawelczyk, Leszek Zabel, Maciej Piotrowska-Kempisty, Hanna Nowicki, Michał Kempisty, Bartosz New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation |
title | New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation |
title_full | New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation |
title_fullStr | New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation |
title_full_unstemmed | New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation |
title_short | New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation |
title_sort | new markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002967/ https://www.ncbi.nlm.nih.gov/pubmed/32016446 http://dx.doi.org/10.3892/mmr.2020.10963 |
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