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Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells
Protocatechualdehyde (PCA) is considered to be the main phenolic component of Phellinus gilvus responsible for its anticancer properties. Previous studies have demonstrated that PCA can have an anticancer effect on multiple cancer types, but little is known about the effect of PCA on melanoma cells....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002996/ https://www.ncbi.nlm.nih.gov/pubmed/31894337 http://dx.doi.org/10.3892/mmr.2019.10896 |
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author | Zhong, Shi Jin, Qinshen Yu, Taihen Zhu, Jianxun Li, Yougui |
author_facet | Zhong, Shi Jin, Qinshen Yu, Taihen Zhu, Jianxun Li, Yougui |
author_sort | Zhong, Shi |
collection | PubMed |
description | Protocatechualdehyde (PCA) is considered to be the main phenolic component of Phellinus gilvus responsible for its anticancer properties. Previous studies have demonstrated that PCA can have an anticancer effect on multiple cancer types, but little is known about the effect of PCA on melanoma cells. The present study investigated the inhibitory abilities and potential anticancer mechanisms of PCA on B16-F10 cells using MTT assay. Cell apoptosis and cell cycle were assessed by flow cytometry using Annexin V-FITC and propidium iodide staining. Whole-transcriptome analysis was used to investigate the effects of PCA on gene expression. PCA significantly decreased cell viability, induced cell cycle arrest at G0/G1 phase and promoted apoptosis of B16-F10 cells, suggesting that PCA could have anticancer effects against melanoma cells. Whole-transcriptome analysis indicated that PCA treatment upregulated genes involved in histone modification and decreased the transcription of genes involved in DNA repair and replication. Kyoto Encyclopedia of Genes and Genomes analysis showed that PCA treatment enhanced the complement and coagulation cascades, and the p53 signaling pathway. The present results indicated that PCA could act as an antitumor agent in melanoma cells, which may provide experimental support for the development of novel therapies to treat melanoma. |
format | Online Article Text |
id | pubmed-7002996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-70029962020-02-12 Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells Zhong, Shi Jin, Qinshen Yu, Taihen Zhu, Jianxun Li, Yougui Mol Med Rep Articles Protocatechualdehyde (PCA) is considered to be the main phenolic component of Phellinus gilvus responsible for its anticancer properties. Previous studies have demonstrated that PCA can have an anticancer effect on multiple cancer types, but little is known about the effect of PCA on melanoma cells. The present study investigated the inhibitory abilities and potential anticancer mechanisms of PCA on B16-F10 cells using MTT assay. Cell apoptosis and cell cycle were assessed by flow cytometry using Annexin V-FITC and propidium iodide staining. Whole-transcriptome analysis was used to investigate the effects of PCA on gene expression. PCA significantly decreased cell viability, induced cell cycle arrest at G0/G1 phase and promoted apoptosis of B16-F10 cells, suggesting that PCA could have anticancer effects against melanoma cells. Whole-transcriptome analysis indicated that PCA treatment upregulated genes involved in histone modification and decreased the transcription of genes involved in DNA repair and replication. Kyoto Encyclopedia of Genes and Genomes analysis showed that PCA treatment enhanced the complement and coagulation cascades, and the p53 signaling pathway. The present results indicated that PCA could act as an antitumor agent in melanoma cells, which may provide experimental support for the development of novel therapies to treat melanoma. D.A. Spandidos 2020-03 2019-12-19 /pmc/articles/PMC7002996/ /pubmed/31894337 http://dx.doi.org/10.3892/mmr.2019.10896 Text en Copyright: © Zhong et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhong, Shi Jin, Qinshen Yu, Taihen Zhu, Jianxun Li, Yougui Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells |
title | Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells |
title_full | Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells |
title_fullStr | Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells |
title_full_unstemmed | Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells |
title_short | Phellinus gilvus-derived protocatechualdehyde induces G0/G1 phase arrest and apoptosis in murine B16-F10 cells |
title_sort | phellinus gilvus-derived protocatechualdehyde induces g0/g1 phase arrest and apoptosis in murine b16-f10 cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002996/ https://www.ncbi.nlm.nih.gov/pubmed/31894337 http://dx.doi.org/10.3892/mmr.2019.10896 |
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