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Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation

The peroxisome proliferator-activated receptor γ (PPARγ) plays an important role in insulin sensitivity and adipocyte differentiation. It is known as ligand-receptor that improves insulin sensitivity in type 2 diabetes mellitus. Several kinds of indigo plant have been already used to treat diabetes...

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Autores principales: Konno, Taisuke, Sasaki, Kenroh, Kobayashi, Kyoko, Murata, Toshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003043/
https://www.ncbi.nlm.nih.gov/pubmed/32016452
http://dx.doi.org/10.3892/mmr.2020.10946
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author Konno, Taisuke
Sasaki, Kenroh
Kobayashi, Kyoko
Murata, Toshihiro
author_facet Konno, Taisuke
Sasaki, Kenroh
Kobayashi, Kyoko
Murata, Toshihiro
author_sort Konno, Taisuke
collection PubMed
description The peroxisome proliferator-activated receptor γ (PPARγ) plays an important role in insulin sensitivity and adipocyte differentiation. It is known as ligand-receptor that improves insulin sensitivity in type 2 diabetes mellitus. Several kinds of indigo plant have been already used to treat diabetes in oriental traditional medicine, but its mechanism has not been clarified yet. To investigate the effect of indirubin, which is a component of Polygonum tinctorium on the cell differentiation and adipprocess in 3T3-L1 cells, 3T3-L1 cells were cultured to determine the effect of cell differentiation and glucose uptake with indirubin. As a result, Indirubin compound enhanced adipocyte differentiation in 3T3-L1 cells similar to rosiglitazone. This effect was terminated by cotreatment with GW9662, a PPARγ antagonist. In mature 3T3-L1 adipocytes, the lipid droplet size and accumulation were reduced by this compound. The basal and insulin-stimulated glucose uptakes were also significantly increased. In addition, indirubin treatment significantly enhanced estrogen level by 1.64-fold with mature adipocytes which can be attributed to its aromatase activity. Conclutionaly, this finding suggested that indirubin is a potential anti-diabetic compound for type 2 diabetes mellitus by promoting adipocyte differentiation and glucose uptake via PPARγ.
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spelling pubmed-70030432020-02-12 Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation Konno, Taisuke Sasaki, Kenroh Kobayashi, Kyoko Murata, Toshihiro Mol Med Rep Articles The peroxisome proliferator-activated receptor γ (PPARγ) plays an important role in insulin sensitivity and adipocyte differentiation. It is known as ligand-receptor that improves insulin sensitivity in type 2 diabetes mellitus. Several kinds of indigo plant have been already used to treat diabetes in oriental traditional medicine, but its mechanism has not been clarified yet. To investigate the effect of indirubin, which is a component of Polygonum tinctorium on the cell differentiation and adipprocess in 3T3-L1 cells, 3T3-L1 cells were cultured to determine the effect of cell differentiation and glucose uptake with indirubin. As a result, Indirubin compound enhanced adipocyte differentiation in 3T3-L1 cells similar to rosiglitazone. This effect was terminated by cotreatment with GW9662, a PPARγ antagonist. In mature 3T3-L1 adipocytes, the lipid droplet size and accumulation were reduced by this compound. The basal and insulin-stimulated glucose uptakes were also significantly increased. In addition, indirubin treatment significantly enhanced estrogen level by 1.64-fold with mature adipocytes which can be attributed to its aromatase activity. Conclutionaly, this finding suggested that indirubin is a potential anti-diabetic compound for type 2 diabetes mellitus by promoting adipocyte differentiation and glucose uptake via PPARγ. D.A. Spandidos 2020-03 2020-01-16 /pmc/articles/PMC7003043/ /pubmed/32016452 http://dx.doi.org/10.3892/mmr.2020.10946 Text en Copyright: © Konno et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Konno, Taisuke
Sasaki, Kenroh
Kobayashi, Kyoko
Murata, Toshihiro
Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation
title Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation
title_full Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation
title_fullStr Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation
title_full_unstemmed Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation
title_short Indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3T3-L1 cells via peroxisome proliferator-activated receptor γ activation
title_sort indirubin promotes adipocyte differentiation and reduces lipid accumulation in 3t3-l1 cells via peroxisome proliferator-activated receptor γ activation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003043/
https://www.ncbi.nlm.nih.gov/pubmed/32016452
http://dx.doi.org/10.3892/mmr.2020.10946
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