Cargando…

Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability

This study aimed to compare the quality of human spermatozoa vitrified by direct plunging into liquid nitrogen vs. liquid air. Spermatozoa were divided into three groups: fresh spermatozoa (Group F) were used as a control. Spermatozoa suspension (20 μl) was vitrified in open granules by direct dropp...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Mengying, Isachenko, Evgenia, Todorov, Plamen, Rahimi, Gohar, Mallmann, Peter, Katkov, Igor I., Isachenko, Vladimir
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003260/ https://www.ncbi.nlm.nih.gov/pubmed/32047807 http://dx.doi.org/10.1155/2020/2934315

Ejemplares similares

Cryoprotectants-Free Vitrification and Conventional Freezing of Human Spermatozoa: A Comparative Transcript Profiling
por: Wang, Mengying, et al.
Publicado: (2022)

Unraveling Subcellular and Ultrastructural Changes During Vitrification of Human Spermatozoa: Effect of a Mitochondria-Targeted Antioxidant and a Permeable Cryoprotectant
por: Kumar, Pradeep, et al.
Publicado: (2021)

Epigenetic Alterations in Cryopreserved Human Spermatozoa: Suspected Potential Functional Defects
por: Wang, Wanxue, et al.
Publicado: (2022)

Whole Ovine Ovaries as a Model for Human: Perfusion with Cryoprotectants In Vivo and In Vitro
por: Isachenko, Vladimir, et al.
Publicado: (2014)

Patient with ovarian insufficiency: baby born after anticancer therapy and re-transplantation of cryopreserved ovarian tissue
por: Isachenko, Vladimir, et al.
Publicado: (2020)

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-xenotransplantation
por: Du, Xinxin, et al.
Publicado: (2020)

High cryo-resistance of SARS-CoV-2 virus: Increased risk of re-contamination at transplantation of cryopreserved ovarian tissue after COVID-19 pandemic
por: Isachenko, Vladimir, et al.
Publicado: (2021)

Long-Time Cooling before Cryopreservation Decreased Translocation of Phosphatidylserine (Ptd-L-Ser) in Human Ovarian Tissue
por: Isachenko, Vladimir, et al.
Publicado: (2015)

Correction: Long-Time Cooling before Cryopreservation Decreased Translocation of Phosphatidylserine (Ptd-L-Ser) in Human Ovarian Tissue
por: Isachenko, Vladimir, et al.
Publicado: (2019)

RNA Transcripts in Human Ovarian Cells: Two-Time Cryopreservation Does Not Affect Developmental Potential
por: Zhou, Yang, et al.
Publicado: (2023)

Comparison of the enzymatic efficiency of Liberase TM and tumor dissociation enzyme: effect on the viability of cells digested from fresh and cryopreserved human ovarian cortex
por: Schmidt, Viola Maria, et al.
Publicado: (2018)

New method of FACS analyzing and sorting of intact whole ovarian fragments (COPAS) after long time (24 h) cooling to 5 °C before cryopreservation
por: Wang, Wanxue, et al.
Publicado: (2021)

Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor
por: Wang, Wanxue, et al.
Publicado: (2022)

Cryopreservation and xenografting of human ovarian fragments: medulla decreases the phosphatidylserine translocation rate
por: Isachenko, Vladimir, et al.
Publicado: (2016)

“Naturalization” of Routine Assisted Reproductive Technologies by In Vitro Culture of Embryos with Microvibration: Sex Ratio, Body Length, and Weight of 2,456 Live-Birth Deliveries after Transfer of 9,624 Embryos In Vitro Cultured in Static System and with Microvibration
por: Isachenko, Vladimir, et al.
Publicado: (2017)

FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa
por: Uribe, Pamela, et al.
Publicado: (2023)

Comparison of In Vitro- and Chorioallantoic Membrane (CAM)-Culture Systems for Cryopreserved Medulla-Contained Human Ovarian Tissue
por: Isachenko, Vladimir, et al.
Publicado: (2012)

Improved cryopreservation of spermatozoa using vitrification: comparison of cryoprotectants and a novel device for long-term storage
por: O’Neill, Helen C., et al.
Publicado: (2019)

Nitrosative stress in human spermatozoa causes cell death characterized by induction of mitochondrial permeability transition-driven necrosis
por: Uribe, Pamela, et al.
Publicado: (2018)

Vitrification Ability of Combined and Single Cryoprotective Agents
por: Faltus, Milos, et al.
Publicado: (2021)

Vitrification of Donkey Sperm: Is It Better Using Permeable Cryoprotectants?
por: Hidalgo, Manuel, et al.
Publicado: (2020)

Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa
por: Alqawasmeh, Odai AM, et al.
Publicado: (2020)

New Alternative Mixtures of Cryoprotectants for Equine Immature Oocyte Vitrification
por: Angel-Velez, Daniel, et al.
Publicado: (2021)

Canine sperm vitrification with nonpermeable cryoprotectants and coconut water extender
por: Antonov, Anton, et al.
Publicado: (2023)

L-proline: a highly effective cryoprotectant for mouse oocyte vitrification
por: Zhang, Lu, et al.
Publicado: (2016)

Effect of Supplementation of Cryoprotectant Solution with Hydroxypropyl Cellulose for Vitrification of Bovine Oocytes
por: Park, Min-Jee, et al.
Publicado: (2022)

Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development
por: Prentice-Biensch, Jennifer R, et al.
Publicado: (2012)

Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration regimens
por: SOMFAI, Tamás, et al.
Publicado: (2015)

Pathogenic Variants in ACTRT1 Cause Acephalic Spermatozoa Syndrome
por: Sha, Yanwei, et al.
Publicado: (2021)

Natural honey acts as a nonpermeating cryoprotectant for promoting bovine oocyte vitrification
por: Alfoteisy, Bilal, et al.
Publicado: (2020)

Interplay of vitrification and ice formation in a cryoprotectant aqueous solution at low temperature
por: Alba-Simionesco, Christiane, et al.
Publicado: (2022)

Cryopreservation of Giraffe Epidydimal Spermatozoa Using Different Extenders and Cryoprotectants
por: Hermes, Robert, et al.
Publicado: (2022)

Permeability of the plasma membrane to water and cryoprotectants in mammalian oocytes and embryos: Its relevance to vitrification
por: Edashige, Keisuke
Publicado: (2016)

Equilibrium vitrification of mouse embryos at various developmental stages using low concentrations of cryoprotectants
por: QIU, Juan, et al.
Publicado: (2021)

Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
por: Jesus, Ana Rita, et al.
Publicado: (2022)

Study of pentoxifylline effects on motility and viability of spermatozoa from infertile asthenozoospermic males
por: Ghasemzadeh, Aliye, et al.
Publicado: (2016)

The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro
por: Halo Jr, Marko, et al.
Publicado: (2021)

Cryopreservation of pig spermatozoa using carboxylated poly-L-lysine as cryoprotectant
por: JIN, Hansol, et al.
Publicado: (2022)

Ultrastructural and Morphalogical Changes of Mouse Ovarian Tissues Following Direct Cover Vitrification with Different Cryoprotectants
por: Ghavami, Maryam, et al.
Publicado: (2015)

Cryopreservation of Endothelial Cells in Various Cryoprotective Agents and Media – Vitrification versus Slow Freezing Methods
por: von Bomhard, Achim, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_3q1lp0cr07e4rpj6o41p0el98n