Highly multiplex guide RNA expression units of CRISPR/Cas9 were completely stable using cosmid amplification in a novel polygonal structure

BACKGROUND: Genome editing using the CRISPR/Cas9 system is now well documented in basic studies and is expected to be applied to gene therapy. Simultaneous expression of multiplex guide RNA (gRNA) and Cas9/Cas9 derivative is attractive for the efficient knockout of genes and a safe double‐nicking st...

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Detalles Bibliográficos
Autores principales: Nakanishi, Tomoko, Maekawa, Aya, Tabata, Hirotaka, Yoshioka, Takashi, Pei, Zheng, Sato, Kumiko, Mori, Mai, Kato, Masaya, Saito, Izumu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003504/
https://www.ncbi.nlm.nih.gov/pubmed/31348845
http://dx.doi.org/10.1002/jgm.3115

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