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Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation

The objective of this study was to identify metabolites within the porcine uterine milieu during the early stages of blastocyst elongation. At Days 9, 10, or 11 of gestation, reproductive tracts of White cross‐bred gilts (n = 38) were collected immediately following harvest and flushed with Roswell...

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Autores principales: Walsh, Sophie C., Miles, Jeremy R., Yao, Linxing, Broeckling, Corey D., Rempel, Lea A., Wright‐Johnson, Elane C., Pannier, Angela K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003770/
https://www.ncbi.nlm.nih.gov/pubmed/31840336
http://dx.doi.org/10.1002/mrd.23306
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author Walsh, Sophie C.
Miles, Jeremy R.
Yao, Linxing
Broeckling, Corey D.
Rempel, Lea A.
Wright‐Johnson, Elane C.
Pannier, Angela K.
author_facet Walsh, Sophie C.
Miles, Jeremy R.
Yao, Linxing
Broeckling, Corey D.
Rempel, Lea A.
Wright‐Johnson, Elane C.
Pannier, Angela K.
author_sort Walsh, Sophie C.
collection PubMed
description The objective of this study was to identify metabolites within the porcine uterine milieu during the early stages of blastocyst elongation. At Days 9, 10, or 11 of gestation, reproductive tracts of White cross‐bred gilts (n = 38) were collected immediately following harvest and flushed with Roswell Park Memorial Institute‐1640 medium. Conceptus morphologies were assessed from each pregnancy and corresponding uterine flushings were assigned to one of five treatment groups based on these morphologies: (a) uniform spherical (n = 8); (b) heterogeneous spherical and ovoid (n = 8); (c) uniform ovoid (n = 8); (d) heterogeneous ovoid and tubular (n = 8); and (e) uniform tubular (n = 6). Uterine flushings from these pregnancies were submitted for nontargeted profiling by gas chromatography–mass spectrometry (GC–MS) and ultra performance liquid chromatography (UPLC)–MS techniques. Unsupervised multivariate principal component analysis (PCA) was performed using pcaMethods and univariate analysis of variance was performed in R with false discovery rate (FDR) adjustment. PCA analysis of the GC–MS and UPLC–MS data identified 153 and 104 metabolites, respectively. After FDR adjustment of the GC–MS and UPLC–MS data, 38 and 59 metabolites, respectively, differed (p < .05) in uterine flushings from pregnancies across the five conceptus stages. Some metabolites were greater (p  < .05) in abundance for uterine flushings containing earlier stage conceptuses (i.e., spherical), such as uric acid, tryptophan, and tyrosine. In contrast, some metabolites were greater (p < .05) in abundance for uterine flushings containing later stage conceptuses (i.e., tubular), such as creatinine, serine, and urea. These data illustrate several putative metabolites that change within the uterine milieu during early porcine blastocyst elongation.
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spelling pubmed-70037702020-02-10 Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation Walsh, Sophie C. Miles, Jeremy R. Yao, Linxing Broeckling, Corey D. Rempel, Lea A. Wright‐Johnson, Elane C. Pannier, Angela K. Mol Reprod Dev Research Articles The objective of this study was to identify metabolites within the porcine uterine milieu during the early stages of blastocyst elongation. At Days 9, 10, or 11 of gestation, reproductive tracts of White cross‐bred gilts (n = 38) were collected immediately following harvest and flushed with Roswell Park Memorial Institute‐1640 medium. Conceptus morphologies were assessed from each pregnancy and corresponding uterine flushings were assigned to one of five treatment groups based on these morphologies: (a) uniform spherical (n = 8); (b) heterogeneous spherical and ovoid (n = 8); (c) uniform ovoid (n = 8); (d) heterogeneous ovoid and tubular (n = 8); and (e) uniform tubular (n = 6). Uterine flushings from these pregnancies were submitted for nontargeted profiling by gas chromatography–mass spectrometry (GC–MS) and ultra performance liquid chromatography (UPLC)–MS techniques. Unsupervised multivariate principal component analysis (PCA) was performed using pcaMethods and univariate analysis of variance was performed in R with false discovery rate (FDR) adjustment. PCA analysis of the GC–MS and UPLC–MS data identified 153 and 104 metabolites, respectively. After FDR adjustment of the GC–MS and UPLC–MS data, 38 and 59 metabolites, respectively, differed (p < .05) in uterine flushings from pregnancies across the five conceptus stages. Some metabolites were greater (p  < .05) in abundance for uterine flushings containing earlier stage conceptuses (i.e., spherical), such as uric acid, tryptophan, and tyrosine. In contrast, some metabolites were greater (p < .05) in abundance for uterine flushings containing later stage conceptuses (i.e., tubular), such as creatinine, serine, and urea. These data illustrate several putative metabolites that change within the uterine milieu during early porcine blastocyst elongation. John Wiley and Sons Inc. 2019-12-16 2020-01 /pmc/articles/PMC7003770/ /pubmed/31840336 http://dx.doi.org/10.1002/mrd.23306 Text en © 2019 The Authors. Molecular Reproduction and Development Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Walsh, Sophie C.
Miles, Jeremy R.
Yao, Linxing
Broeckling, Corey D.
Rempel, Lea A.
Wright‐Johnson, Elane C.
Pannier, Angela K.
Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
title Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
title_full Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
title_fullStr Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
title_full_unstemmed Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
title_short Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
title_sort metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003770/
https://www.ncbi.nlm.nih.gov/pubmed/31840336
http://dx.doi.org/10.1002/mrd.23306
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