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LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation
Late endosomal/lysosomal adaptor and MAPK and mTOR activator (LAMTOR/Ragulator) is a scaffold protein complex that anchors and regulates multiprotein signaling units on late endosomes/lysosomes. To identify LAMTOR‐modulated endolysosomal proteins, primary macrophages were derived from bone marrow of...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003824/ https://www.ncbi.nlm.nih.gov/pubmed/31423582 http://dx.doi.org/10.1002/1873-3468.13579 |
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author | Lamberti, Giorgia De Smet, Cedric H. Angelova, Mihaela Kremser, Leopold Taub, Nicole Herrmann, Caroline Hess, Michael W. Rainer, Johannes Tancevski, Ivan Schweigreiter, Rüdiger Kofler, Reinhard Schmiedinger, Thomas Vietor, Ilja Trajanoski, Zlatko Ejsing, Christer S. Lindner, Herbert H. Huber, Lukas A. Stasyk, Taras |
author_facet | Lamberti, Giorgia De Smet, Cedric H. Angelova, Mihaela Kremser, Leopold Taub, Nicole Herrmann, Caroline Hess, Michael W. Rainer, Johannes Tancevski, Ivan Schweigreiter, Rüdiger Kofler, Reinhard Schmiedinger, Thomas Vietor, Ilja Trajanoski, Zlatko Ejsing, Christer S. Lindner, Herbert H. Huber, Lukas A. Stasyk, Taras |
author_sort | Lamberti, Giorgia |
collection | PubMed |
description | Late endosomal/lysosomal adaptor and MAPK and mTOR activator (LAMTOR/Ragulator) is a scaffold protein complex that anchors and regulates multiprotein signaling units on late endosomes/lysosomes. To identify LAMTOR‐modulated endolysosomal proteins, primary macrophages were derived from bone marrow of conditional knockout mice carrying a specific deletion of LAMTOR2 in the monocyte/macrophage cell lineage. Affymetrix‐based transcriptomic analysis and quantitative iTRAQ‐based organelle proteomic analysis of endosomes derived from macrophages were performed. Further analyses showed that LAMTOR could be a novel regulator of foam cell differentiation. The lipid droplet formation phenotype observed in macrophages was additionally confirmed in MEFs, where lipidomic analysis identified cholesterol esters as specifically downregulated in LAMTOR2 knockout cells. The data obtained indicate a function of LAMTOR2 in lipid metabolism. |
format | Online Article Text |
id | pubmed-7003824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70038242020-02-10 LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation Lamberti, Giorgia De Smet, Cedric H. Angelova, Mihaela Kremser, Leopold Taub, Nicole Herrmann, Caroline Hess, Michael W. Rainer, Johannes Tancevski, Ivan Schweigreiter, Rüdiger Kofler, Reinhard Schmiedinger, Thomas Vietor, Ilja Trajanoski, Zlatko Ejsing, Christer S. Lindner, Herbert H. Huber, Lukas A. Stasyk, Taras FEBS Lett Research Letters Late endosomal/lysosomal adaptor and MAPK and mTOR activator (LAMTOR/Ragulator) is a scaffold protein complex that anchors and regulates multiprotein signaling units on late endosomes/lysosomes. To identify LAMTOR‐modulated endolysosomal proteins, primary macrophages were derived from bone marrow of conditional knockout mice carrying a specific deletion of LAMTOR2 in the monocyte/macrophage cell lineage. Affymetrix‐based transcriptomic analysis and quantitative iTRAQ‐based organelle proteomic analysis of endosomes derived from macrophages were performed. Further analyses showed that LAMTOR could be a novel regulator of foam cell differentiation. The lipid droplet formation phenotype observed in macrophages was additionally confirmed in MEFs, where lipidomic analysis identified cholesterol esters as specifically downregulated in LAMTOR2 knockout cells. The data obtained indicate a function of LAMTOR2 in lipid metabolism. John Wiley and Sons Inc. 2019-08-26 2020-01 /pmc/articles/PMC7003824/ /pubmed/31423582 http://dx.doi.org/10.1002/1873-3468.13579 Text en © 2019 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Letters Lamberti, Giorgia De Smet, Cedric H. Angelova, Mihaela Kremser, Leopold Taub, Nicole Herrmann, Caroline Hess, Michael W. Rainer, Johannes Tancevski, Ivan Schweigreiter, Rüdiger Kofler, Reinhard Schmiedinger, Thomas Vietor, Ilja Trajanoski, Zlatko Ejsing, Christer S. Lindner, Herbert H. Huber, Lukas A. Stasyk, Taras LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation |
title | LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation |
title_full | LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation |
title_fullStr | LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation |
title_full_unstemmed | LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation |
title_short | LAMTOR/Ragulator regulates lipid metabolism in macrophages and foam cell differentiation |
title_sort | lamtor/ragulator regulates lipid metabolism in macrophages and foam cell differentiation |
topic | Research Letters |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003824/ https://www.ncbi.nlm.nih.gov/pubmed/31423582 http://dx.doi.org/10.1002/1873-3468.13579 |
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