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Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii

Flagella are the well‐known structural appendages used by bacteria for motility. Although generally reported to be non‐motile, the enteropathogenic bacterial species Escherichia albertii produces flagella intermittently. We found that E. albertii expressed flagella under specific environmental condi...

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Autores principales: Murakami, Koichi, Kimura, Shinya, Nagafuchi, Osamu, Sekizuka, Tsuyoshi, Onozuka, Daisuke, Mizukoshi, Fuminori, Tsukagoshi, Hiroyuki, Ishioka, Taisei, Asai, Tetsuo, Hirai, Shinichiro, Musashi, Manami, Suzuki, Motoi, Ohnishi, Makoto, Oishi, Kazunori, Saruki, Nobuhiro, Kimura, Hirokazu, Iyoda, Sunao, Kuroda, Makoto, Fujimoto, Shuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003939/
https://www.ncbi.nlm.nih.gov/pubmed/31845481
http://dx.doi.org/10.1111/1758-2229.12818
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author Murakami, Koichi
Kimura, Shinya
Nagafuchi, Osamu
Sekizuka, Tsuyoshi
Onozuka, Daisuke
Mizukoshi, Fuminori
Tsukagoshi, Hiroyuki
Ishioka, Taisei
Asai, Tetsuo
Hirai, Shinichiro
Musashi, Manami
Suzuki, Motoi
Ohnishi, Makoto
Oishi, Kazunori
Saruki, Nobuhiro
Kimura, Hirokazu
Iyoda, Sunao
Kuroda, Makoto
Fujimoto, Shuji
author_facet Murakami, Koichi
Kimura, Shinya
Nagafuchi, Osamu
Sekizuka, Tsuyoshi
Onozuka, Daisuke
Mizukoshi, Fuminori
Tsukagoshi, Hiroyuki
Ishioka, Taisei
Asai, Tetsuo
Hirai, Shinichiro
Musashi, Manami
Suzuki, Motoi
Ohnishi, Makoto
Oishi, Kazunori
Saruki, Nobuhiro
Kimura, Hirokazu
Iyoda, Sunao
Kuroda, Makoto
Fujimoto, Shuji
author_sort Murakami, Koichi
collection PubMed
description Flagella are the well‐known structural appendages used by bacteria for motility. Although generally reported to be non‐motile, the enteropathogenic bacterial species Escherichia albertii produces flagella intermittently. We found that E. albertii expressed flagella under specific environmental conditions. After several generations (involving 4 to 12‐h incubations), six of the twelve strains we investigated displayed swimming motility in various aquatic environments, including pond water containing nutrients from pigeon droppings (10% suspension) as well as in 20 × −diluted tryptic soy broth. The most significant motility determinant was a temperature between 15 and 30 °C. At 20 °C in the 10% pigeon‐dropping suspension, microscopic observations revealed that some cells (1%–95% of six strains) showed swimming motility. Electron microscopy showed that the E. albertii cells expressed flagella. Lower concentrations of some substrates (including nutrients) may be of secondary importance for E. albertii flagella expression. Interestingly, the non‐motile strains (n = 6/12) contained pseudogenes corresponding to essential flagella structural proteins. After being released from its host into surface water, E. albertii may express flagella to move toward nutrient sources or new hosts.
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spelling pubmed-70039392020-02-11 Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii Murakami, Koichi Kimura, Shinya Nagafuchi, Osamu Sekizuka, Tsuyoshi Onozuka, Daisuke Mizukoshi, Fuminori Tsukagoshi, Hiroyuki Ishioka, Taisei Asai, Tetsuo Hirai, Shinichiro Musashi, Manami Suzuki, Motoi Ohnishi, Makoto Oishi, Kazunori Saruki, Nobuhiro Kimura, Hirokazu Iyoda, Sunao Kuroda, Makoto Fujimoto, Shuji Environ Microbiol Rep Brief Reports Flagella are the well‐known structural appendages used by bacteria for motility. Although generally reported to be non‐motile, the enteropathogenic bacterial species Escherichia albertii produces flagella intermittently. We found that E. albertii expressed flagella under specific environmental conditions. After several generations (involving 4 to 12‐h incubations), six of the twelve strains we investigated displayed swimming motility in various aquatic environments, including pond water containing nutrients from pigeon droppings (10% suspension) as well as in 20 × −diluted tryptic soy broth. The most significant motility determinant was a temperature between 15 and 30 °C. At 20 °C in the 10% pigeon‐dropping suspension, microscopic observations revealed that some cells (1%–95% of six strains) showed swimming motility. Electron microscopy showed that the E. albertii cells expressed flagella. Lower concentrations of some substrates (including nutrients) may be of secondary importance for E. albertii flagella expression. Interestingly, the non‐motile strains (n = 6/12) contained pseudogenes corresponding to essential flagella structural proteins. After being released from its host into surface water, E. albertii may express flagella to move toward nutrient sources or new hosts. John Wiley & Sons, Inc. 2019-12-25 2020-02 /pmc/articles/PMC7003939/ /pubmed/31845481 http://dx.doi.org/10.1111/1758-2229.12818 Text en © 2019 The Authors. Environmental Microbiology Reports published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Reports
Murakami, Koichi
Kimura, Shinya
Nagafuchi, Osamu
Sekizuka, Tsuyoshi
Onozuka, Daisuke
Mizukoshi, Fuminori
Tsukagoshi, Hiroyuki
Ishioka, Taisei
Asai, Tetsuo
Hirai, Shinichiro
Musashi, Manami
Suzuki, Motoi
Ohnishi, Makoto
Oishi, Kazunori
Saruki, Nobuhiro
Kimura, Hirokazu
Iyoda, Sunao
Kuroda, Makoto
Fujimoto, Shuji
Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii
title Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii
title_full Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii
title_fullStr Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii
title_full_unstemmed Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii
title_short Flagellum expression and swimming activity by the zoonotic pathogen Escherichia albertii
title_sort flagellum expression and swimming activity by the zoonotic pathogen escherichia albertii
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003939/
https://www.ncbi.nlm.nih.gov/pubmed/31845481
http://dx.doi.org/10.1111/1758-2229.12818
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