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A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal
Seed vigour and early establishment are important factors determining the yield of crops. A wheat nitrate‐inducible NAC transcription factor, TaNAC2, plays a critical role in promoting crop growth and nitrogen use efficiency (NUE), and now its role in seed vigour is revealed. A TaNAC2 regulated gene...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004088/ https://www.ncbi.nlm.nih.gov/pubmed/31581317 http://dx.doi.org/10.1111/nph.16234 |
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author | Li, Wenjing He, Xue Chen, Yi Jing, Yanfu Shen, Chuncai Yang, Junbo Teng, Wan Zhao, Xueqiang Hu, Weijuan Hu, Mengyun Li, Hui Miller, Anthony J. Tong, Yiping |
author_facet | Li, Wenjing He, Xue Chen, Yi Jing, Yanfu Shen, Chuncai Yang, Junbo Teng, Wan Zhao, Xueqiang Hu, Weijuan Hu, Mengyun Li, Hui Miller, Anthony J. Tong, Yiping |
author_sort | Li, Wenjing |
collection | PubMed |
description | Seed vigour and early establishment are important factors determining the yield of crops. A wheat nitrate‐inducible NAC transcription factor, TaNAC2, plays a critical role in promoting crop growth and nitrogen use efficiency (NUE), and now its role in seed vigour is revealed. A TaNAC2 regulated gene was identified that is a NRT2‐type nitrate transporter TaNRT2.5 with a key role in seed vigour. Overexpressing TaNAC2‐5A increases grain nitrate concentration and seed vigour by directly binding to the promoter of TaNRT2.5‐3B and positively regulating its expression. TaNRT2.5 is expressed in developing grain, particularly the embryo and husk. In Xenopus oocyte assays TaNRT2.5 requires a partner protein TaNAR2.1 to give nitrate transport activity, and the transporter locates to the tonoplast in a tobacco leaf transient expression system. Furthermore, in the root TaNRT2.5 and TaNRT2.1 function in post‐anthesis acquisition of soil nitrate. Overexpression of TaNRT2.5‐3B increases seed vigour, grain nitrate concentration and yield, whereas RNA interference of TaNRT2.5 has the opposite effects. The TaNAC2‐NRT2.5 module has a key role in regulating grain nitrate accumulation and seed vigour. Both genes can potentially be used to improve grain yield and NUE in wheat. |
format | Online Article Text |
id | pubmed-7004088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70040882020-02-11 A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal Li, Wenjing He, Xue Chen, Yi Jing, Yanfu Shen, Chuncai Yang, Junbo Teng, Wan Zhao, Xueqiang Hu, Weijuan Hu, Mengyun Li, Hui Miller, Anthony J. Tong, Yiping New Phytol Research Seed vigour and early establishment are important factors determining the yield of crops. A wheat nitrate‐inducible NAC transcription factor, TaNAC2, plays a critical role in promoting crop growth and nitrogen use efficiency (NUE), and now its role in seed vigour is revealed. A TaNAC2 regulated gene was identified that is a NRT2‐type nitrate transporter TaNRT2.5 with a key role in seed vigour. Overexpressing TaNAC2‐5A increases grain nitrate concentration and seed vigour by directly binding to the promoter of TaNRT2.5‐3B and positively regulating its expression. TaNRT2.5 is expressed in developing grain, particularly the embryo and husk. In Xenopus oocyte assays TaNRT2.5 requires a partner protein TaNAR2.1 to give nitrate transport activity, and the transporter locates to the tonoplast in a tobacco leaf transient expression system. Furthermore, in the root TaNRT2.5 and TaNRT2.1 function in post‐anthesis acquisition of soil nitrate. Overexpression of TaNRT2.5‐3B increases seed vigour, grain nitrate concentration and yield, whereas RNA interference of TaNRT2.5 has the opposite effects. The TaNAC2‐NRT2.5 module has a key role in regulating grain nitrate accumulation and seed vigour. Both genes can potentially be used to improve grain yield and NUE in wheat. John Wiley and Sons Inc. 2019-11-13 2020-02 /pmc/articles/PMC7004088/ /pubmed/31581317 http://dx.doi.org/10.1111/nph.16234 Text en © 2019 The Authors New Phytologist © 2019 New Phytologist Trust This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Li, Wenjing He, Xue Chen, Yi Jing, Yanfu Shen, Chuncai Yang, Junbo Teng, Wan Zhao, Xueqiang Hu, Weijuan Hu, Mengyun Li, Hui Miller, Anthony J. Tong, Yiping A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal |
title | A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal |
title_full | A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal |
title_fullStr | A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal |
title_full_unstemmed | A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal |
title_short | A wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal |
title_sort | wheat transcription factor positively sets seed vigour by regulating the grain nitrate signal |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004088/ https://www.ncbi.nlm.nih.gov/pubmed/31581317 http://dx.doi.org/10.1111/nph.16234 |
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