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Dynamic acetylation profile during mammalian neurulation
BACKGROUND: Neural tube defects (NTDs) result from failure of neural tube closure during embryogenesis. These severe birth defects of the central nervous system include anencephaly and spina bifida, and affect 0.5–2 per 1,000 pregnancies worldwide in humans. It has been demonstrated that acetylation...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004172/ https://www.ncbi.nlm.nih.gov/pubmed/31758757 http://dx.doi.org/10.1002/bdr2.1618 |
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author | Massa, Valentina Avagliano, Laura Grazioli, Paolo De Castro, Sandra C. P. Parodi, Chiara Savery, Dawn Vergani, Patrizia Cuttin, Serena Doi, Patrizia Bulfamante, Gaetano Copp, Andrew J. Greene, Nicholas D. E. |
author_facet | Massa, Valentina Avagliano, Laura Grazioli, Paolo De Castro, Sandra C. P. Parodi, Chiara Savery, Dawn Vergani, Patrizia Cuttin, Serena Doi, Patrizia Bulfamante, Gaetano Copp, Andrew J. Greene, Nicholas D. E. |
author_sort | Massa, Valentina |
collection | PubMed |
description | BACKGROUND: Neural tube defects (NTDs) result from failure of neural tube closure during embryogenesis. These severe birth defects of the central nervous system include anencephaly and spina bifida, and affect 0.5–2 per 1,000 pregnancies worldwide in humans. It has been demonstrated that acetylation plays a pivotal role during neural tube closure, as animal models for defective histone acetyltransferase proteins display NTDs. Acetylation represents an important component of the complex network of posttranslational regulatory interactions, suggesting a possible fundamental role during primary neurulation events. This study aimed to assess protein acetylation contribution to early patterning of the central nervous system both in human and murine specimens. METHODS: We used both human and mouse (Cited2 (−/−)) samples to analyze the dynamic acetylation of proteins during embryo development through immunohistochemistry, western blot analysis and quantitative polymerase chain reaction. RESULTS: We report the dynamic profile of histone and protein acetylation status during neural tube closure. We also report a rescue effect in an animal model by chemical p53 inhibition. CONCLUSIONS: Our data suggest that the p53‐acetylation equilibrium may play a role in primary neurulation in mammals. |
format | Online Article Text |
id | pubmed-7004172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70041722020-02-11 Dynamic acetylation profile during mammalian neurulation Massa, Valentina Avagliano, Laura Grazioli, Paolo De Castro, Sandra C. P. Parodi, Chiara Savery, Dawn Vergani, Patrizia Cuttin, Serena Doi, Patrizia Bulfamante, Gaetano Copp, Andrew J. Greene, Nicholas D. E. Birth Defects Res Brief Report BACKGROUND: Neural tube defects (NTDs) result from failure of neural tube closure during embryogenesis. These severe birth defects of the central nervous system include anencephaly and spina bifida, and affect 0.5–2 per 1,000 pregnancies worldwide in humans. It has been demonstrated that acetylation plays a pivotal role during neural tube closure, as animal models for defective histone acetyltransferase proteins display NTDs. Acetylation represents an important component of the complex network of posttranslational regulatory interactions, suggesting a possible fundamental role during primary neurulation events. This study aimed to assess protein acetylation contribution to early patterning of the central nervous system both in human and murine specimens. METHODS: We used both human and mouse (Cited2 (−/−)) samples to analyze the dynamic acetylation of proteins during embryo development through immunohistochemistry, western blot analysis and quantitative polymerase chain reaction. RESULTS: We report the dynamic profile of histone and protein acetylation status during neural tube closure. We also report a rescue effect in an animal model by chemical p53 inhibition. CONCLUSIONS: Our data suggest that the p53‐acetylation equilibrium may play a role in primary neurulation in mammals. John Wiley & Sons, Inc. 2019-11-23 2020-01-15 /pmc/articles/PMC7004172/ /pubmed/31758757 http://dx.doi.org/10.1002/bdr2.1618 Text en © 2019 The Authors. Birth Defects Research published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Massa, Valentina Avagliano, Laura Grazioli, Paolo De Castro, Sandra C. P. Parodi, Chiara Savery, Dawn Vergani, Patrizia Cuttin, Serena Doi, Patrizia Bulfamante, Gaetano Copp, Andrew J. Greene, Nicholas D. E. Dynamic acetylation profile during mammalian neurulation |
title | Dynamic acetylation profile during mammalian neurulation |
title_full | Dynamic acetylation profile during mammalian neurulation |
title_fullStr | Dynamic acetylation profile during mammalian neurulation |
title_full_unstemmed | Dynamic acetylation profile during mammalian neurulation |
title_short | Dynamic acetylation profile during mammalian neurulation |
title_sort | dynamic acetylation profile during mammalian neurulation |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004172/ https://www.ncbi.nlm.nih.gov/pubmed/31758757 http://dx.doi.org/10.1002/bdr2.1618 |
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