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Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila

Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and i...

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Detalles Bibliográficos
Autores principales: Koyama, Leslie Ann Jaramillo, Aranda-Díaz, Andrés, Su, Yu-Han, Balachandra, Shruthi, Martin, Judy Lisette, Ludington, William B., Huang, Kerwyn Casey, O’Brien, Lucy Erin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004386/
https://www.ncbi.nlm.nih.gov/pubmed/31986129
http://dx.doi.org/10.1371/journal.pbio.3000567
Descripción
Sumario:Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount’s capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.