An adaptive excitation source for high speed multiphoton microscopy

Optical imaging at high spatial and temporal resolution is important to understand brain function. We demonstrate an adaptive femtosecond excitation source that only illuminates the region of interest. We show that the source reduces the power requirement for two- or three-photon imaging of brain ac...

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Detalles Bibliográficos
Autores principales: Li, Bo, Wu, Chunyan, Wang, Mengran, Charan, Kriti, Xu, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004891/
https://www.ncbi.nlm.nih.gov/pubmed/31792434
http://dx.doi.org/10.1038/s41592-019-0663-9
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author Li, Bo
Wu, Chunyan
Wang, Mengran
Charan, Kriti
Xu, Chris
author_facet Li, Bo
Wu, Chunyan
Wang, Mengran
Charan, Kriti
Xu, Chris
author_sort Li, Bo
collection PubMed
description Optical imaging at high spatial and temporal resolution is important to understand brain function. We demonstrate an adaptive femtosecond excitation source that only illuminates the region of interest. We show that the source reduces the power requirement for two- or three-photon imaging of brain activity in awake mice by more than 30 times. The adaptive excitation source represents a new direction in the development of high speed imaging systems.
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spelling pubmed-70048912020-06-02 An adaptive excitation source for high speed multiphoton microscopy Li, Bo Wu, Chunyan Wang, Mengran Charan, Kriti Xu, Chris Nat Methods Article Optical imaging at high spatial and temporal resolution is important to understand brain function. We demonstrate an adaptive femtosecond excitation source that only illuminates the region of interest. We show that the source reduces the power requirement for two- or three-photon imaging of brain activity in awake mice by more than 30 times. The adaptive excitation source represents a new direction in the development of high speed imaging systems. 2019-12-02 2020-02 /pmc/articles/PMC7004891/ /pubmed/31792434 http://dx.doi.org/10.1038/s41592-019-0663-9 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Li, Bo
Wu, Chunyan
Wang, Mengran
Charan, Kriti
Xu, Chris
An adaptive excitation source for high speed multiphoton microscopy
title An adaptive excitation source for high speed multiphoton microscopy
title_full An adaptive excitation source for high speed multiphoton microscopy
title_fullStr An adaptive excitation source for high speed multiphoton microscopy
title_full_unstemmed An adaptive excitation source for high speed multiphoton microscopy
title_short An adaptive excitation source for high speed multiphoton microscopy
title_sort adaptive excitation source for high speed multiphoton microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004891/
https://www.ncbi.nlm.nih.gov/pubmed/31792434
http://dx.doi.org/10.1038/s41592-019-0663-9
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