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Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development

As one of the best-studied RNA binding proteins in plant, pentatricopeptide repeats (PPRs) protein are mainly targeted to mitochondria and/or chloroplasts for RNA processing to regulate the biogenesis and function of the organelles, but its molecular mechanism and role in development remain to be fu...

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Autores principales: Zhao, Peng, Wang, Fang, Li, Na, Shi, Dong-Qiao, Yang, Wei-Cai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005036/
https://www.ncbi.nlm.nih.gov/pubmed/32029763
http://dx.doi.org/10.1038/s41598-020-58495-5
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author Zhao, Peng
Wang, Fang
Li, Na
Shi, Dong-Qiao
Yang, Wei-Cai
author_facet Zhao, Peng
Wang, Fang
Li, Na
Shi, Dong-Qiao
Yang, Wei-Cai
author_sort Zhao, Peng
collection PubMed
description As one of the best-studied RNA binding proteins in plant, pentatricopeptide repeats (PPRs) protein are mainly targeted to mitochondria and/or chloroplasts for RNA processing to regulate the biogenesis and function of the organelles, but its molecular mechanism and role in development remain to be further revealed. Here, we identified a mitochondria-localized P-type small PPR protein, MITOCHONDRION-MEDIATED GROWTH DEFECT 1 (MID1) that is crucial for Arabidopsis development. Mutation in MID1 causes retarded embryo development and stunted plant growth with defects in cell expansion and proliferation. Molecular experiments showed that MID1 is required for the splicing of the nad2 intron 1 in mitochondria. Consistently, mid1 plants display significant reduction in the abundance and activity of mitochondrial respiration complex I, accompanied by abnormal mitochondrial morphology and energy metabolism. Furthermore, MID1 is associated with other trans-factors involved in NICOTINAMIDE ADENINE DINUCLEOTIDE HYDROGEN (NADH) DEHYDROGENASE SUBUNIT 2 (nad2) intron 1 splicing, and interacts directly with itself and MITOCHONDRIAL STABILITY FACTOR 1 (MTSF1). This suggests that MID1 most likely functions as a dimer for nad2 intron 1 splicing. Together, we characterized a novel PPR protein MID1 for nad2 intron 1 splicing.
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spelling pubmed-70050362020-02-14 Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development Zhao, Peng Wang, Fang Li, Na Shi, Dong-Qiao Yang, Wei-Cai Sci Rep Article As one of the best-studied RNA binding proteins in plant, pentatricopeptide repeats (PPRs) protein are mainly targeted to mitochondria and/or chloroplasts for RNA processing to regulate the biogenesis and function of the organelles, but its molecular mechanism and role in development remain to be further revealed. Here, we identified a mitochondria-localized P-type small PPR protein, MITOCHONDRION-MEDIATED GROWTH DEFECT 1 (MID1) that is crucial for Arabidopsis development. Mutation in MID1 causes retarded embryo development and stunted plant growth with defects in cell expansion and proliferation. Molecular experiments showed that MID1 is required for the splicing of the nad2 intron 1 in mitochondria. Consistently, mid1 plants display significant reduction in the abundance and activity of mitochondrial respiration complex I, accompanied by abnormal mitochondrial morphology and energy metabolism. Furthermore, MID1 is associated with other trans-factors involved in NICOTINAMIDE ADENINE DINUCLEOTIDE HYDROGEN (NADH) DEHYDROGENASE SUBUNIT 2 (nad2) intron 1 splicing, and interacts directly with itself and MITOCHONDRIAL STABILITY FACTOR 1 (MTSF1). This suggests that MID1 most likely functions as a dimer for nad2 intron 1 splicing. Together, we characterized a novel PPR protein MID1 for nad2 intron 1 splicing. Nature Publishing Group UK 2020-02-06 /pmc/articles/PMC7005036/ /pubmed/32029763 http://dx.doi.org/10.1038/s41598-020-58495-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhao, Peng
Wang, Fang
Li, Na
Shi, Dong-Qiao
Yang, Wei-Cai
Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_full Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_fullStr Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_full_unstemmed Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_short Pentatricopeptide repeat protein MID1 modulates nad2 intron 1 splicing and Arabidopsis development
title_sort pentatricopeptide repeat protein mid1 modulates nad2 intron 1 splicing and arabidopsis development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005036/
https://www.ncbi.nlm.nih.gov/pubmed/32029763
http://dx.doi.org/10.1038/s41598-020-58495-5
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