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Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption
Protein hydrolysates are an important part of the human diet. Often, they are prepared from milk, soy, or collagen. In the present study, four different collagen hydrolysates were tested, varying in the average molecular weight and the animal source. Three types of samples, the dissolved start produ...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005076/ https://www.ncbi.nlm.nih.gov/pubmed/31872275 http://dx.doi.org/10.1007/s00216-019-02323-x |
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author | Kleinnijenhuis, Anne J. van Holthoon, Frédérique L. Maathuis, Annet J.H. Vanhoecke, Barbara Prawitt, Janne Wauquier, Fabien Wittrant, Yohann |
author_facet | Kleinnijenhuis, Anne J. van Holthoon, Frédérique L. Maathuis, Annet J.H. Vanhoecke, Barbara Prawitt, Janne Wauquier, Fabien Wittrant, Yohann |
author_sort | Kleinnijenhuis, Anne J. |
collection | PubMed |
description | Protein hydrolysates are an important part of the human diet. Often, they are prepared from milk, soy, or collagen. In the present study, four different collagen hydrolysates were tested, varying in the average molecular weight and the animal source. Three types of samples, the dissolved start products, in vitro generated dialysates (containing the digested components that are potentially available for small intestinal absorption), and human serum collected after product ingestion, were analyzed using LC-MS to compare the state of the hydrolysates before and after absorption, i.e., uptake into the blood. It was found that the composition of the collagen hydrolysates prior to and after ingestion was highly complex and dynamic, which made it challenging to predefine a strategy for a targeted analysis. Therefore, we implemented a new analytical approach to first map hydrolysate data sets by performing non-targeted LC-MS analysis followed by non-targeted and targeted data analysis. It was shown that the insight gained by following such a top down (data) analytical workflow could be crucial for defining a suitable targeted setup and considering data trends beyond the defined targets. After having defined and performed a limited targeted analysis, it was found that, in our experimental setup, Hyp-Gly and especially Pro-Hyp contributed significantly as carrier to the total Hyp increase in blood after ingestion of collagen hydrolysate. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-02323-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7005076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-70050762020-02-25 Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption Kleinnijenhuis, Anne J. van Holthoon, Frédérique L. Maathuis, Annet J.H. Vanhoecke, Barbara Prawitt, Janne Wauquier, Fabien Wittrant, Yohann Anal Bioanal Chem Research Paper Protein hydrolysates are an important part of the human diet. Often, they are prepared from milk, soy, or collagen. In the present study, four different collagen hydrolysates were tested, varying in the average molecular weight and the animal source. Three types of samples, the dissolved start products, in vitro generated dialysates (containing the digested components that are potentially available for small intestinal absorption), and human serum collected after product ingestion, were analyzed using LC-MS to compare the state of the hydrolysates before and after absorption, i.e., uptake into the blood. It was found that the composition of the collagen hydrolysates prior to and after ingestion was highly complex and dynamic, which made it challenging to predefine a strategy for a targeted analysis. Therefore, we implemented a new analytical approach to first map hydrolysate data sets by performing non-targeted LC-MS analysis followed by non-targeted and targeted data analysis. It was shown that the insight gained by following such a top down (data) analytical workflow could be crucial for defining a suitable targeted setup and considering data trends beyond the defined targets. After having defined and performed a limited targeted analysis, it was found that, in our experimental setup, Hyp-Gly and especially Pro-Hyp contributed significantly as carrier to the total Hyp increase in blood after ingestion of collagen hydrolysate. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00216-019-02323-x) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-12-24 2020 /pmc/articles/PMC7005076/ /pubmed/31872275 http://dx.doi.org/10.1007/s00216-019-02323-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Paper Kleinnijenhuis, Anne J. van Holthoon, Frédérique L. Maathuis, Annet J.H. Vanhoecke, Barbara Prawitt, Janne Wauquier, Fabien Wittrant, Yohann Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption |
title | Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption |
title_full | Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption |
title_fullStr | Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption |
title_full_unstemmed | Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption |
title_short | Non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption |
title_sort | non-targeted and targeted analysis of collagen hydrolysates during the course of digestion and absorption |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005076/ https://www.ncbi.nlm.nih.gov/pubmed/31872275 http://dx.doi.org/10.1007/s00216-019-02323-x |
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