Cargando…

Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis

PIN-FORMED (PIN) auxin efflux carriers with a long central hydrophilic loop (long PINs) have been implicated in organogenesis. However, the role of short hydrophilic loop PINs (short PINs) in organogenesis is largely unknown. In this study, we investigated the role of a short PIN, PIN8, in lateral r...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Hyodong, Ganguly, Anindya, Lee, Richard Dongwook, Park, Minho, Cho, Hyung-Taeg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005106/
https://www.ncbi.nlm.nih.gov/pubmed/32082353
http://dx.doi.org/10.3389/fpls.2019.01808
_version_ 1783494862683766784
author Lee, Hyodong
Ganguly, Anindya
Lee, Richard Dongwook
Park, Minho
Cho, Hyung-Taeg
author_facet Lee, Hyodong
Ganguly, Anindya
Lee, Richard Dongwook
Park, Minho
Cho, Hyung-Taeg
author_sort Lee, Hyodong
collection PubMed
description PIN-FORMED (PIN) auxin efflux carriers with a long central hydrophilic loop (long PINs) have been implicated in organogenesis. However, the role of short hydrophilic loop PINs (short PINs) in organogenesis is largely unknown. In this study, we investigated the role of a short PIN, PIN8, in lateral root (LR) development in Arabidopsis thaliana. The loss-of-function mutation in PIN8 significantly decreased LR density, mostly by affecting the emergence stage. PIN8 showed a sporadic expression pattern along the root vascular cells in the phloem, where the PIN8 protein predominantly localized to intracellular compartments. During LR primordium development, PIN8 was expressed at the late stage. Plasma membrane (PM)-localized long PINs suppressed LR formation when expressed in the PIN8 domain. Conversely, an auxin influx carrier, AUX1, restored the wild-type (WT) LR density when expressed in the PIN8 domain of the pin8 mutant root. Moreover, LR emergence was considerably inhibited when AXR2-1, the dominant negative form of Aux/IAA7, compromised auxin signaling in the PIN8 domain. Consistent with these observations, the expression of many genes implicated in late LR development was suppressed in the pin8 mutant compared with the WT. Our results suggest that the intracellularly localized PIN8 affects LR development most likely by modulating intracellular auxin translocation. Thus, the function of PIN8 is distinctive from that of PM-localized long PINs, where they generate local auxin gradients for organogenesis by conducting cell-to-cell auxin reflux.
format Online
Article
Text
id pubmed-7005106
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-70051062020-02-20 Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis Lee, Hyodong Ganguly, Anindya Lee, Richard Dongwook Park, Minho Cho, Hyung-Taeg Front Plant Sci Plant Science PIN-FORMED (PIN) auxin efflux carriers with a long central hydrophilic loop (long PINs) have been implicated in organogenesis. However, the role of short hydrophilic loop PINs (short PINs) in organogenesis is largely unknown. In this study, we investigated the role of a short PIN, PIN8, in lateral root (LR) development in Arabidopsis thaliana. The loss-of-function mutation in PIN8 significantly decreased LR density, mostly by affecting the emergence stage. PIN8 showed a sporadic expression pattern along the root vascular cells in the phloem, where the PIN8 protein predominantly localized to intracellular compartments. During LR primordium development, PIN8 was expressed at the late stage. Plasma membrane (PM)-localized long PINs suppressed LR formation when expressed in the PIN8 domain. Conversely, an auxin influx carrier, AUX1, restored the wild-type (WT) LR density when expressed in the PIN8 domain of the pin8 mutant root. Moreover, LR emergence was considerably inhibited when AXR2-1, the dominant negative form of Aux/IAA7, compromised auxin signaling in the PIN8 domain. Consistent with these observations, the expression of many genes implicated in late LR development was suppressed in the pin8 mutant compared with the WT. Our results suggest that the intracellularly localized PIN8 affects LR development most likely by modulating intracellular auxin translocation. Thus, the function of PIN8 is distinctive from that of PM-localized long PINs, where they generate local auxin gradients for organogenesis by conducting cell-to-cell auxin reflux. Frontiers Media S.A. 2020-01-31 /pmc/articles/PMC7005106/ /pubmed/32082353 http://dx.doi.org/10.3389/fpls.2019.01808 Text en Copyright © 2020 Lee, Ganguly, Lee, Park and Cho http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lee, Hyodong
Ganguly, Anindya
Lee, Richard Dongwook
Park, Minho
Cho, Hyung-Taeg
Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis
title Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis
title_full Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis
title_fullStr Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis
title_full_unstemmed Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis
title_short Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence in Arabidopsis
title_sort intracellularly localized pin-formed8 promotes lateral root emergence in arabidopsis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005106/
https://www.ncbi.nlm.nih.gov/pubmed/32082353
http://dx.doi.org/10.3389/fpls.2019.01808
work_keys_str_mv AT leehyodong intracellularlylocalizedpinformed8promoteslateralrootemergenceinarabidopsis
AT gangulyanindya intracellularlylocalizedpinformed8promoteslateralrootemergenceinarabidopsis
AT leericharddongwook intracellularlylocalizedpinformed8promoteslateralrootemergenceinarabidopsis
AT parkminho intracellularlylocalizedpinformed8promoteslateralrootemergenceinarabidopsis
AT chohyungtaeg intracellularlylocalizedpinformed8promoteslateralrootemergenceinarabidopsis