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Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1

Soybean is a major crop that is used as a source of vegetable oil for human use. To develop transgenic soybean with high α-linolenic acid (ALA; 18:3) content, the FAD3-1 gene isolated from lesquerella (Physaria fendleri) was used to construct vectors with two different seed-specific promoters, soybe...

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Autores principales: Yeom, Wan Woo, Kim, Hye Jeong, Lee, Kyeong-Ryeol, Cho, Hyun Suk, Kim, Jin-Young, Jung, Ho Won, Oh, Seon-Woo, Jun, Sang Eun, Kim, Hyun Uk, Chung, Young-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005135/
https://www.ncbi.nlm.nih.gov/pubmed/32082356
http://dx.doi.org/10.3389/fpls.2019.01812
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author Yeom, Wan Woo
Kim, Hye Jeong
Lee, Kyeong-Ryeol
Cho, Hyun Suk
Kim, Jin-Young
Jung, Ho Won
Oh, Seon-Woo
Jun, Sang Eun
Kim, Hyun Uk
Chung, Young-Soo
author_facet Yeom, Wan Woo
Kim, Hye Jeong
Lee, Kyeong-Ryeol
Cho, Hyun Suk
Kim, Jin-Young
Jung, Ho Won
Oh, Seon-Woo
Jun, Sang Eun
Kim, Hyun Uk
Chung, Young-Soo
author_sort Yeom, Wan Woo
collection PubMed
description Soybean is a major crop that is used as a source of vegetable oil for human use. To develop transgenic soybean with high α-linolenic acid (ALA; 18:3) content, the FAD3-1 gene isolated from lesquerella (Physaria fendleri) was used to construct vectors with two different seed-specific promoters, soybean β-conglycinin (Pβ-con) and kidney bean phaseolin (Pphas), and one constitutive cauliflower mosaic virus 35S promoter (P35S). The corresponding vectors were used for Agrobacterium-mediated transformation of imbibed mature half seeds. The transformation efficiency was approximately 2%, 1%, and 3% and 21, 7, and 17 transgenic plants were produced, respectively. T-DNA insertion and expression of the transgene were confirmed from most of the transgenic plants by polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), reverse transcription PCR (RT-PCR), and Southern blot analysis. The fatty acid composition of soybean seeds was analyzed by gas chromatography. The 18:3 content in the transgenic generation T(1) seeds was increased 7-fold in Pβ-con:PfFAD3-1, 4-fold in Pphas : PfFAD3-1, and 1.6-fold in P35S:PfFAD3-1 compared to the 18:3 content in soybean “Kwangankong”. The increased content of 18:3 in the Pβ-con:PfFAD3-1 soybean (T(1)) resulted in a 52.6% increase in total fatty acids, with a larger decrease in 18:1 content than 18:2 content. The increase in 18:3 content was also maintained and reached 42% in the Pphas : PfFAD3-1 transgenic generation T(2). Investigations of the agronomic traits of 12 Pβ-con:PfFAD3-1 transgenic lines (T(1)) revealed that plant height, number of branches, nodes, pods, total seeds, and total seed weight were significantly higher in several transgenic lines than those in non-transgenic soybean. Especially, an increase in seed size was observed upon expression of the PfFAD3-1 gene with the β-conglycinin promoter, and 6%–14% higher seed lengths were measured from the transgenic lines.
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spelling pubmed-70051352020-02-20 Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1 Yeom, Wan Woo Kim, Hye Jeong Lee, Kyeong-Ryeol Cho, Hyun Suk Kim, Jin-Young Jung, Ho Won Oh, Seon-Woo Jun, Sang Eun Kim, Hyun Uk Chung, Young-Soo Front Plant Sci Plant Science Soybean is a major crop that is used as a source of vegetable oil for human use. To develop transgenic soybean with high α-linolenic acid (ALA; 18:3) content, the FAD3-1 gene isolated from lesquerella (Physaria fendleri) was used to construct vectors with two different seed-specific promoters, soybean β-conglycinin (Pβ-con) and kidney bean phaseolin (Pphas), and one constitutive cauliflower mosaic virus 35S promoter (P35S). The corresponding vectors were used for Agrobacterium-mediated transformation of imbibed mature half seeds. The transformation efficiency was approximately 2%, 1%, and 3% and 21, 7, and 17 transgenic plants were produced, respectively. T-DNA insertion and expression of the transgene were confirmed from most of the transgenic plants by polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), reverse transcription PCR (RT-PCR), and Southern blot analysis. The fatty acid composition of soybean seeds was analyzed by gas chromatography. The 18:3 content in the transgenic generation T(1) seeds was increased 7-fold in Pβ-con:PfFAD3-1, 4-fold in Pphas : PfFAD3-1, and 1.6-fold in P35S:PfFAD3-1 compared to the 18:3 content in soybean “Kwangankong”. The increased content of 18:3 in the Pβ-con:PfFAD3-1 soybean (T(1)) resulted in a 52.6% increase in total fatty acids, with a larger decrease in 18:1 content than 18:2 content. The increase in 18:3 content was also maintained and reached 42% in the Pphas : PfFAD3-1 transgenic generation T(2). Investigations of the agronomic traits of 12 Pβ-con:PfFAD3-1 transgenic lines (T(1)) revealed that plant height, number of branches, nodes, pods, total seeds, and total seed weight were significantly higher in several transgenic lines than those in non-transgenic soybean. Especially, an increase in seed size was observed upon expression of the PfFAD3-1 gene with the β-conglycinin promoter, and 6%–14% higher seed lengths were measured from the transgenic lines. Frontiers Media S.A. 2020-01-31 /pmc/articles/PMC7005135/ /pubmed/32082356 http://dx.doi.org/10.3389/fpls.2019.01812 Text en Copyright © 2020 Yeom, Kim, Lee, Cho, Kim, Jung, Oh, Jun, Kim and Chung http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yeom, Wan Woo
Kim, Hye Jeong
Lee, Kyeong-Ryeol
Cho, Hyun Suk
Kim, Jin-Young
Jung, Ho Won
Oh, Seon-Woo
Jun, Sang Eun
Kim, Hyun Uk
Chung, Young-Soo
Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
title Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
title_full Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
title_fullStr Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
title_full_unstemmed Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
title_short Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
title_sort increased production of α-linolenic acid in soybean seeds by overexpression of lesquerella fad3-1
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005135/
https://www.ncbi.nlm.nih.gov/pubmed/32082356
http://dx.doi.org/10.3389/fpls.2019.01812
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