Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy

Reversibly switchable fluorescent proteins (RSFPs) serve as markers in advanced fluorescence imaging. Photoswitching from a non-fluorescent off-state to a fluorescent on-state involves trans-to-cis chromophore isomerization and proton transfer. Whereas excited-state events on the ps timescale have b...

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Detalles Bibliográficos
Autores principales: Woodhouse, Joyce, Nass Kovacs, Gabriela, Coquelle, Nicolas, Uriarte, Lucas M., Adam, Virgile, Barends, Thomas R. M., Byrdin, Martin, de la Mora, Eugenio, Bruce Doak, R., Feliks, Mikolaj, Field, Martin, Fieschi, Franck, Guillon, Virginia, Jakobs, Stefan, Joti, Yasumasa, Macheboeuf, Pauline, Motomura, Koji, Nass, Karol, Owada, Shigeki, Roome, Christopher M., Ruckebusch, Cyril, Schirò, Giorgio, Shoeman, Robert L., Thepaut, Michel, Togashi, Tadashi, Tono, Kensuke, Yabashi, Makina, Cammarata, Marco, Foucar, Lutz, Bourgeois, Dominique, Sliwa, Michel, Colletier, Jacques-Philippe, Schlichting, Ilme, Weik, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005145/
https://www.ncbi.nlm.nih.gov/pubmed/32029745
http://dx.doi.org/10.1038/s41467-020-14537-0

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