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Expression of protocadherin-20 in mouse taste buds

Taste information is detected by taste cells and then transmitted to the brain through the taste nerve fibers. According to our previous data, there may be specific coding of taste quality between taste cells and nerve fibers. However, the molecular mechanisms underlying this coding specificity rema...

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Autores principales: Hirose, Fumie, Takai, Shingo, Takahashi, Ichiro, Shigemura, Noriatsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005180/
https://www.ncbi.nlm.nih.gov/pubmed/32029864
http://dx.doi.org/10.1038/s41598-020-58991-8
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author Hirose, Fumie
Takai, Shingo
Takahashi, Ichiro
Shigemura, Noriatsu
author_facet Hirose, Fumie
Takai, Shingo
Takahashi, Ichiro
Shigemura, Noriatsu
author_sort Hirose, Fumie
collection PubMed
description Taste information is detected by taste cells and then transmitted to the brain through the taste nerve fibers. According to our previous data, there may be specific coding of taste quality between taste cells and nerve fibers. However, the molecular mechanisms underlying this coding specificity remain unclear. The purpose of this study was to identify candidate molecules that may regulate the specific coding. GeneChip analysis of mRNA isolated from the mice taste papillae and taste ganglia revealed that 14 members of the cadherin superfamily, which are important regulators of synapse formation and plasticity, were expressed in both tissues. Among them, protocadherin-20 (Pcdh20) was highly expressed in a subset of taste bud cells, and co-expressed with taste receptor type 1 member 3 (T1R3, a marker of sweet- or umami-sensitive taste cells) but not gustducin or carbonic anhydrase-4 (markers of bitter/sweet- and sour-sensitive taste cells, respectively) in circumvallate papillae. Furthermore, Pcdh20 expression in taste cells occurred later than T1R3 expression during the morphogenesis of taste papillae. Thus, Pcdh20 may be involved in taste quality-specific connections between differentiated taste cells and their partner neurons, thereby acting as a molecular tag for the coding of sweet and/or umami taste.
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spelling pubmed-70051802020-02-18 Expression of protocadherin-20 in mouse taste buds Hirose, Fumie Takai, Shingo Takahashi, Ichiro Shigemura, Noriatsu Sci Rep Article Taste information is detected by taste cells and then transmitted to the brain through the taste nerve fibers. According to our previous data, there may be specific coding of taste quality between taste cells and nerve fibers. However, the molecular mechanisms underlying this coding specificity remain unclear. The purpose of this study was to identify candidate molecules that may regulate the specific coding. GeneChip analysis of mRNA isolated from the mice taste papillae and taste ganglia revealed that 14 members of the cadherin superfamily, which are important regulators of synapse formation and plasticity, were expressed in both tissues. Among them, protocadherin-20 (Pcdh20) was highly expressed in a subset of taste bud cells, and co-expressed with taste receptor type 1 member 3 (T1R3, a marker of sweet- or umami-sensitive taste cells) but not gustducin or carbonic anhydrase-4 (markers of bitter/sweet- and sour-sensitive taste cells, respectively) in circumvallate papillae. Furthermore, Pcdh20 expression in taste cells occurred later than T1R3 expression during the morphogenesis of taste papillae. Thus, Pcdh20 may be involved in taste quality-specific connections between differentiated taste cells and their partner neurons, thereby acting as a molecular tag for the coding of sweet and/or umami taste. Nature Publishing Group UK 2020-02-06 /pmc/articles/PMC7005180/ /pubmed/32029864 http://dx.doi.org/10.1038/s41598-020-58991-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hirose, Fumie
Takai, Shingo
Takahashi, Ichiro
Shigemura, Noriatsu
Expression of protocadherin-20 in mouse taste buds
title Expression of protocadherin-20 in mouse taste buds
title_full Expression of protocadherin-20 in mouse taste buds
title_fullStr Expression of protocadherin-20 in mouse taste buds
title_full_unstemmed Expression of protocadherin-20 in mouse taste buds
title_short Expression of protocadherin-20 in mouse taste buds
title_sort expression of protocadherin-20 in mouse taste buds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005180/
https://www.ncbi.nlm.nih.gov/pubmed/32029864
http://dx.doi.org/10.1038/s41598-020-58991-8
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