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Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain

Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here...

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Autores principales: Burt, Alister, Cassidy, C. Keith, Ames, Peter, Bacia-Verloop, Maria, Baulard, Megghane, Huard, Karine, Luthey-Schulten, Zaida, Desfosses, Ambroise, Stansfeld, Phillip J., Margolin, William, Parkinson, John S., Gutsche, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005262/
https://www.ncbi.nlm.nih.gov/pubmed/32029744
http://dx.doi.org/10.1038/s41467-020-14350-9
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author Burt, Alister
Cassidy, C. Keith
Ames, Peter
Bacia-Verloop, Maria
Baulard, Megghane
Huard, Karine
Luthey-Schulten, Zaida
Desfosses, Ambroise
Stansfeld, Phillip J.
Margolin, William
Parkinson, John S.
Gutsche, Irina
author_facet Burt, Alister
Cassidy, C. Keith
Ames, Peter
Bacia-Verloop, Maria
Baulard, Megghane
Huard, Karine
Luthey-Schulten, Zaida
Desfosses, Ambroise
Stansfeld, Phillip J.
Margolin, William
Parkinson, John S.
Gutsche, Irina
author_sort Burt, Alister
collection PubMed
description Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here we introduce an Escherichia coli strain that forms small minicells possessing extended and highly ordered chemosensory arrays. We use cryo-electron tomography and subtomogram averaging to provide a three-dimensional map of a complete core signalling unit, with visible densities corresponding to the HAMP and periplasmic domains. This map, combined with previously determined high resolution structures and molecular dynamics simulations, yields a molecular model of the transmembrane core signalling unit and enables spatial localisation of its individual domains. Our work thus offers a solid structural basis for the interpretation of a wide range of existing data and the design of further experiments to elucidate signalling mechanisms within the core signalling unit and larger array.
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spelling pubmed-70052622020-02-10 Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain Burt, Alister Cassidy, C. Keith Ames, Peter Bacia-Verloop, Maria Baulard, Megghane Huard, Karine Luthey-Schulten, Zaida Desfosses, Ambroise Stansfeld, Phillip J. Margolin, William Parkinson, John S. Gutsche, Irina Nat Commun Article Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays. Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit. Here we introduce an Escherichia coli strain that forms small minicells possessing extended and highly ordered chemosensory arrays. We use cryo-electron tomography and subtomogram averaging to provide a three-dimensional map of a complete core signalling unit, with visible densities corresponding to the HAMP and periplasmic domains. This map, combined with previously determined high resolution structures and molecular dynamics simulations, yields a molecular model of the transmembrane core signalling unit and enables spatial localisation of its individual domains. Our work thus offers a solid structural basis for the interpretation of a wide range of existing data and the design of further experiments to elucidate signalling mechanisms within the core signalling unit and larger array. Nature Publishing Group UK 2020-02-06 /pmc/articles/PMC7005262/ /pubmed/32029744 http://dx.doi.org/10.1038/s41467-020-14350-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Burt, Alister
Cassidy, C. Keith
Ames, Peter
Bacia-Verloop, Maria
Baulard, Megghane
Huard, Karine
Luthey-Schulten, Zaida
Desfosses, Ambroise
Stansfeld, Phillip J.
Margolin, William
Parkinson, John S.
Gutsche, Irina
Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
title Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
title_full Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
title_fullStr Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
title_full_unstemmed Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
title_short Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain
title_sort complete structure of the chemosensory array core signalling unit in an e. coli minicell strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005262/
https://www.ncbi.nlm.nih.gov/pubmed/32029744
http://dx.doi.org/10.1038/s41467-020-14350-9
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