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Linescan microscopy data to extract diffusion coefficient of a fluorescent species using a commercial confocal microscope

We report here on the measurement of the diffusion coefficient of fluorescent species using a commercial microscope possessing a resonant scanner. Sequential linescans with a rate of up to 12 kHz yield a temporal resolution of 83 μs, making the setup amenable to measure diffusion rates over a range...

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Detalles Bibliográficos
Autores principales: Bathe-Peters, Marc, Gmach, Philipp, Annibale, Paolo, Lohse, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005367/
https://www.ncbi.nlm.nih.gov/pubmed/32055652
http://dx.doi.org/10.1016/j.dib.2019.105063