Cargando…
MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor
In recent years, it has been reported that non-coding RNAs, especially microRNAs (miRNAs) and long non-coding RNAs, act as melanogenesis-regulating molecules in melanocytes. We found that the expression levels of miR-141-3p and miR-200a-3p were decreased significantly by α-melanocyte-stimulating hor...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005774/ https://www.ncbi.nlm.nih.gov/pubmed/32034251 http://dx.doi.org/10.1038/s41598-020-58911-w |
_version_ | 1783495008055197696 |
---|---|
author | Itoh, Tomohiro Fukatani, Kanako Nakashima, Ayaka Suzuki, Kengo |
author_facet | Itoh, Tomohiro Fukatani, Kanako Nakashima, Ayaka Suzuki, Kengo |
author_sort | Itoh, Tomohiro |
collection | PubMed |
description | In recent years, it has been reported that non-coding RNAs, especially microRNAs (miRNAs) and long non-coding RNAs, act as melanogenesis-regulating molecules in melanocytes. We found that the expression levels of miR-141-3p and miR-200a-3p were decreased significantly by α-melanocyte-stimulating hormone (α-MSH) stimulation in mouse melanocyte B16-4A5 cells, as demonstrated by a miRNA array. Overexpression of miR-141-3p and miR-200a-3p in B16-4A5 cells suppressed melanogenesis and tyrosinase activity. Moreover, both miR-141-3p and miR-200a-3p showed direct targeting of microphthalmia-associated transcription factor using a luciferase reporter assay. Furthermore, topical transfection of miR-141-3p and miR-200a-3p to three-dimensional reconstructed human skin tissue inhibited α-MSH-stimulated melanin biosynthesis. Taken together, our findings indicate that downregulation of miR-141-3p and miR-200a-3p during the α-MSH-stimulated melanogenesis process acts as an important intrinsic signal. This result is expected to lead to the development of miRNA-based whitening therapeutics. |
format | Online Article Text |
id | pubmed-7005774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70057742020-02-18 MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor Itoh, Tomohiro Fukatani, Kanako Nakashima, Ayaka Suzuki, Kengo Sci Rep Article In recent years, it has been reported that non-coding RNAs, especially microRNAs (miRNAs) and long non-coding RNAs, act as melanogenesis-regulating molecules in melanocytes. We found that the expression levels of miR-141-3p and miR-200a-3p were decreased significantly by α-melanocyte-stimulating hormone (α-MSH) stimulation in mouse melanocyte B16-4A5 cells, as demonstrated by a miRNA array. Overexpression of miR-141-3p and miR-200a-3p in B16-4A5 cells suppressed melanogenesis and tyrosinase activity. Moreover, both miR-141-3p and miR-200a-3p showed direct targeting of microphthalmia-associated transcription factor using a luciferase reporter assay. Furthermore, topical transfection of miR-141-3p and miR-200a-3p to three-dimensional reconstructed human skin tissue inhibited α-MSH-stimulated melanin biosynthesis. Taken together, our findings indicate that downregulation of miR-141-3p and miR-200a-3p during the α-MSH-stimulated melanogenesis process acts as an important intrinsic signal. This result is expected to lead to the development of miRNA-based whitening therapeutics. Nature Publishing Group UK 2020-02-07 /pmc/articles/PMC7005774/ /pubmed/32034251 http://dx.doi.org/10.1038/s41598-020-58911-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Itoh, Tomohiro Fukatani, Kanako Nakashima, Ayaka Suzuki, Kengo MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor |
title | MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor |
title_full | MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor |
title_fullStr | MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor |
title_full_unstemmed | MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor |
title_short | MicroRNA-141-3p and microRNA-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor |
title_sort | microrna-141-3p and microrna-200a-3p regulate α-melanocyte stimulating hormone-stimulated melanogenesis by directly targeting microphthalmia-associated transcription factor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005774/ https://www.ncbi.nlm.nih.gov/pubmed/32034251 http://dx.doi.org/10.1038/s41598-020-58911-w |
work_keys_str_mv | AT itohtomohiro microrna1413pandmicrorna200a3pregulateamelanocytestimulatinghormonestimulatedmelanogenesisbydirectlytargetingmicrophthalmiaassociatedtranscriptionfactor AT fukatanikanako microrna1413pandmicrorna200a3pregulateamelanocytestimulatinghormonestimulatedmelanogenesisbydirectlytargetingmicrophthalmiaassociatedtranscriptionfactor AT nakashimaayaka microrna1413pandmicrorna200a3pregulateamelanocytestimulatinghormonestimulatedmelanogenesisbydirectlytargetingmicrophthalmiaassociatedtranscriptionfactor AT suzukikengo microrna1413pandmicrorna200a3pregulateamelanocytestimulatinghormonestimulatedmelanogenesisbydirectlytargetingmicrophthalmiaassociatedtranscriptionfactor |