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RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA
RNase H1 is able to recognize DNA/RNA heteroduplexes and to degrade their RNA component. As a consequence, it has been implicated in different aspects of mtDNA replication such as primer formation, primer removal, and replication termination, and significant differences have been reported between co...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006045/ https://www.ncbi.nlm.nih.gov/pubmed/32082360 http://dx.doi.org/10.3389/fgene.2019.01393 |
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author | Reyes, Aurelio Rusecka, Joanna Tońska, Katarzyna Zeviani, Massimo |
author_facet | Reyes, Aurelio Rusecka, Joanna Tońska, Katarzyna Zeviani, Massimo |
author_sort | Reyes, Aurelio |
collection | PubMed |
description | RNase H1 is able to recognize DNA/RNA heteroduplexes and to degrade their RNA component. As a consequence, it has been implicated in different aspects of mtDNA replication such as primer formation, primer removal, and replication termination, and significant differences have been reported between control and mutant RNASEH1 skin fibroblasts from patients. However, neither mtDNA depletion nor the presence of deletions have been described in skin fibroblasts while still presenting signs of mitochondrial dysfunction (lower mitochondrial membrane potential, reduced oxygen consumption, slow growth in galactose). Here, we show that RNase H1 has an effect on mtDNA transcripts, most likely through the regulation of 7S RNA and other R-loops. The observed effect on both mitochondrial mRNAs and 16S rRNA results in decreased mitochondrial translation and subsequently mitochondrial dysfunction in cells carrying mutations in RNASEH1. |
format | Online Article Text |
id | pubmed-7006045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70060452020-02-20 RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA Reyes, Aurelio Rusecka, Joanna Tońska, Katarzyna Zeviani, Massimo Front Genet Genetics RNase H1 is able to recognize DNA/RNA heteroduplexes and to degrade their RNA component. As a consequence, it has been implicated in different aspects of mtDNA replication such as primer formation, primer removal, and replication termination, and significant differences have been reported between control and mutant RNASEH1 skin fibroblasts from patients. However, neither mtDNA depletion nor the presence of deletions have been described in skin fibroblasts while still presenting signs of mitochondrial dysfunction (lower mitochondrial membrane potential, reduced oxygen consumption, slow growth in galactose). Here, we show that RNase H1 has an effect on mtDNA transcripts, most likely through the regulation of 7S RNA and other R-loops. The observed effect on both mitochondrial mRNAs and 16S rRNA results in decreased mitochondrial translation and subsequently mitochondrial dysfunction in cells carrying mutations in RNASEH1. Frontiers Media S.A. 2020-01-31 /pmc/articles/PMC7006045/ /pubmed/32082360 http://dx.doi.org/10.3389/fgene.2019.01393 Text en Copyright © 2020 Reyes, Rusecka, Tońska and Zeviani http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Reyes, Aurelio Rusecka, Joanna Tońska, Katarzyna Zeviani, Massimo RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA |
title | RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA |
title_full | RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA |
title_fullStr | RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA |
title_full_unstemmed | RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA |
title_short | RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA |
title_sort | rnase h1 regulates mitochondrial transcription and translation via the degradation of 7s rna |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006045/ https://www.ncbi.nlm.nih.gov/pubmed/32082360 http://dx.doi.org/10.3389/fgene.2019.01393 |
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