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High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

BACKGROUND: pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it. On the basis of pWB980, constructing an E. coli–B. subtilis s...

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Detalles Bibliográficos
Autores principales: Zhao, XingYa, Xu, JianYong, Tan, Ming, Zhen, Jie, Shu, WenJu, Yang, ShiBin, Ma, YanHe, Zheng, HongChen, Song, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006159/
https://www.ncbi.nlm.nih.gov/pubmed/32028973
http://dx.doi.org/10.1186/s12934-020-1296-5