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In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors
Smoke flavors based on the thermal decomposition of wood have been applied to a variety of food products as an alternative for traditional smoking. Despite its increasing use, the available genotoxicity data on wood smoke flavors (WSF) are still controversial. Thus, potential genotoxic effects of WS...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006255/ https://www.ncbi.nlm.nih.gov/pubmed/32038811 http://dx.doi.org/10.5487/TR.2008.24.4.321 |
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author | Chung, Young-Shin Ahn, Jun-Ho Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Kim, Yun-Ji Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Lee, Michael |
author_facet | Chung, Young-Shin Ahn, Jun-Ho Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Kim, Yun-Ji Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Lee, Michael |
author_sort | Chung, Young-Shin |
collection | PubMed |
description | Smoke flavors based on the thermal decomposition of wood have been applied to a variety of food products as an alternative for traditional smoking. Despite its increasing use, the available genotoxicity data on wood smoke flavors (WSF) are still controversial. Thus, potential genotoxic effects of WSF in four short-term in vitro genotoxicity assays were investigated, which included the Ames assay, chromosomal aberration assay, micronucleus test and the alkaline comet assay. WSF did not cause any mutation in the Ames assay using five tester strains at six concentrations of 0.16, 0.31, 0.63, 1.25, 2.5 and 5 µl/plate. To assess clastogenic effect, the in vitro chromosomal aberration assay was performed using Chinese hamster lung cells. No statistically significant increase in the number of metaphases with structural aberrations was observed at the concentrations of 1.25, 2.5, and 5 µl/ml. The in vitro comet assay and micronucleus test results obtained on L5178Y cells also revealed that WSF has no genotoxicity potential, although there was a marginal increase in micronuclei frequencies and DNA damage in the respective micronucleus and comet assays. Taken together, based on the results obtained from these four in vitro studies, it is concluded that WSF is not a mutagenic agent in bacterial cells and causes no chromosomal and DNA damage in mammalian cells in vitro. |
format | Online Article Text |
id | pubmed-7006255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-70062552020-02-07 In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors Chung, Young-Shin Ahn, Jun-Ho Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Kim, Yun-Ji Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Lee, Michael Toxicol Res Article Smoke flavors based on the thermal decomposition of wood have been applied to a variety of food products as an alternative for traditional smoking. Despite its increasing use, the available genotoxicity data on wood smoke flavors (WSF) are still controversial. Thus, potential genotoxic effects of WSF in four short-term in vitro genotoxicity assays were investigated, which included the Ames assay, chromosomal aberration assay, micronucleus test and the alkaline comet assay. WSF did not cause any mutation in the Ames assay using five tester strains at six concentrations of 0.16, 0.31, 0.63, 1.25, 2.5 and 5 µl/plate. To assess clastogenic effect, the in vitro chromosomal aberration assay was performed using Chinese hamster lung cells. No statistically significant increase in the number of metaphases with structural aberrations was observed at the concentrations of 1.25, 2.5, and 5 µl/ml. The in vitro comet assay and micronucleus test results obtained on L5178Y cells also revealed that WSF has no genotoxicity potential, although there was a marginal increase in micronuclei frequencies and DNA damage in the respective micronucleus and comet assays. Taken together, based on the results obtained from these four in vitro studies, it is concluded that WSF is not a mutagenic agent in bacterial cells and causes no chromosomal and DNA damage in mammalian cells in vitro. Springer Singapore 2008-12-01 2008-12 /pmc/articles/PMC7006255/ /pubmed/32038811 http://dx.doi.org/10.5487/TR.2008.24.4.321 Text en © Korean Society of Toxicology 2008 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chung, Young-Shin Ahn, Jun-Ho Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Kim, Yun-Ji Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Lee, Michael In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors |
title | In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors |
title_full | In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors |
title_fullStr | In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors |
title_full_unstemmed | In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors |
title_short | In Vitro Studies on the Genotoxic Effects of Wood Smoke Flavors |
title_sort | in vitro studies on the genotoxic effects of wood smoke flavors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006255/ https://www.ncbi.nlm.nih.gov/pubmed/32038811 http://dx.doi.org/10.5487/TR.2008.24.4.321 |
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