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Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays
Carrageenan is a naturally-occurring sulfated polygalactan which has been widely used in the dairy industry and a gelling agent in non-dairy products. In this study, four short-term in vitro genotoxicity assays were investigated to evaluate the potential genotoxic effects of carrageenan. The mutagen...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006343/ https://www.ncbi.nlm.nih.gov/pubmed/32038820 http://dx.doi.org/10.5487/TR.2009.25.1.051 |
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author | Chung, Young-Shin Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Seo, Young-Rok Lee, Michael |
author_facet | Chung, Young-Shin Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Seo, Young-Rok Lee, Michael |
author_sort | Chung, Young-Shin |
collection | PubMed |
description | Carrageenan is a naturally-occurring sulfated polygalactan which has been widely used in the dairy industry and a gelling agent in non-dairy products. In this study, four short-term in vitro genotoxicity assays were investigated to evaluate the potential genotoxic effects of carrageenan. The mutagenic-ity of carrageenan was evaluated up to a maximum dose of 5 mg/plate in Ames test. There was no increase in the number of revertant colonies compared to its negative control at any dose in all of strains tested. To assess clastogenic effect, the in vitro chromosomal aberration assay was performed using Chinese hamster lung cells. Carrageenan was not considered to be clastogenic in this assay at up to the highest feasible concentration which could be evaluated. The in vitro comet assay and micronucleus test results obtained on L5178Y cells also revealed that carrageenan has no genotoxicity potential, although there was a marginal increase in micronuclei frequencies and DNA damage in the respective micronucleus and comet assays. Taken together, our results indicate that carrageenan was not genotoxic based on four in vitro genotoxicity results. |
format | Online Article Text |
id | pubmed-7006343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-70063432020-02-07 Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays Chung, Young-Shin Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Seo, Young-Rok Lee, Michael Toxicol Res Article Carrageenan is a naturally-occurring sulfated polygalactan which has been widely used in the dairy industry and a gelling agent in non-dairy products. In this study, four short-term in vitro genotoxicity assays were investigated to evaluate the potential genotoxic effects of carrageenan. The mutagenic-ity of carrageenan was evaluated up to a maximum dose of 5 mg/plate in Ames test. There was no increase in the number of revertant colonies compared to its negative control at any dose in all of strains tested. To assess clastogenic effect, the in vitro chromosomal aberration assay was performed using Chinese hamster lung cells. Carrageenan was not considered to be clastogenic in this assay at up to the highest feasible concentration which could be evaluated. The in vitro comet assay and micronucleus test results obtained on L5178Y cells also revealed that carrageenan has no genotoxicity potential, although there was a marginal increase in micronuclei frequencies and DNA damage in the respective micronucleus and comet assays. Taken together, our results indicate that carrageenan was not genotoxic based on four in vitro genotoxicity results. Springer Singapore 2009-03-01 2009-03 /pmc/articles/PMC7006343/ /pubmed/32038820 http://dx.doi.org/10.5487/TR.2009.25.1.051 Text en © Korean Society of Toxicology 2009 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Chung, Young-Shin Eum, Ki-Hwan Choi, Seon-A Oh, Se-Wook Park, Sue Nie Yum, Young-Na Kim, Joo-Hwan Seo, Young-Rok Lee, Michael Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays |
title | Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays |
title_full | Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays |
title_fullStr | Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays |
title_full_unstemmed | Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays |
title_short | Genotoxicity Studies on Carrageenan: Short-term In Vitro Assays |
title_sort | genotoxicity studies on carrageenan: short-term in vitro assays |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006343/ https://www.ncbi.nlm.nih.gov/pubmed/32038820 http://dx.doi.org/10.5487/TR.2009.25.1.051 |
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