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Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds

CONTEXT: Burn therapy (MEBT)/moist exposed burn ointment (MEBO) is an effective traditional Chinese medicine method to treat diabetic wound, but the mechanism is unclear. Autophagy has been proved to be closely related with wound healing, so MEBO/MEBT is hypothesized to promote diabetic wound healin...

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Autores principales: Zheng, Aitian, Ma, Huadan, Liu, Xianbin, Huang, Qing, Li, Zheng, Wang, Lin, Zeng, Na, Wu, Biaoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006791/
https://www.ncbi.nlm.nih.gov/pubmed/31967912
http://dx.doi.org/10.1080/13880209.2019.1711430
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author Zheng, Aitian
Ma, Huadan
Liu, Xianbin
Huang, Qing
Li, Zheng
Wang, Lin
Zeng, Na
Wu, Biaoliang
author_facet Zheng, Aitian
Ma, Huadan
Liu, Xianbin
Huang, Qing
Li, Zheng
Wang, Lin
Zeng, Na
Wu, Biaoliang
author_sort Zheng, Aitian
collection PubMed
description CONTEXT: Burn therapy (MEBT)/moist exposed burn ointment (MEBO) is an effective traditional Chinese medicine method to treat diabetic wound, but the mechanism is unclear. Autophagy has been proved to be closely related with wound healing, so MEBO/MEBT is hypothesized to promote diabetic wound healing by regulating autophagy. OBJECTIVE: To explore the mechanism of moist exposed MEBT/MEBO promoting diabetic wound repair. MATERIALS AND METHODS: Eighty male Wistar rats were randomly assigned to control (n = 20) and diabetic group induced by intraperitoneal injection of STZ (n = 60), which were further randomly assigned to MEBO, Kangfuxin and model groups (n = 20 each). All rats underwent full-thickness skin resection in the back. Wound healing was dynamically observed and wound tissues were collected at five time points for pathological examination, autophagosome and the expression of PI3K, Akt and mTOR. RESULTS: The healing time in the control group was the shortest, no statistically significant difference was found between the MEBO and the Kangfuxin group (p = 0.76). The morphology of autophagosomes ranged large to small, which was the most obvious in the MEBO group. The mRNA and protein expression of PI3K, Akt and mTOR in each group reached the peak on Day 5, the levels in the MEBO group were the highest (F = 18.43, 19.97, 15.36, p < 0.05). On Day 11, the expression levels in each group began to decline. DISCUSSION AND CONCLUSIONS: In this study, we discussed the molecular mechanism of MEBT/MEBO promoting the repair of diabetic ulcer wounds through autophagy and PI3K-Akt-mTOR signalling pathway, which provides a new way for drug design in the future.
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spelling pubmed-70067912020-02-20 Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds Zheng, Aitian Ma, Huadan Liu, Xianbin Huang, Qing Li, Zheng Wang, Lin Zeng, Na Wu, Biaoliang Pharm Biol Research Article CONTEXT: Burn therapy (MEBT)/moist exposed burn ointment (MEBO) is an effective traditional Chinese medicine method to treat diabetic wound, but the mechanism is unclear. Autophagy has been proved to be closely related with wound healing, so MEBO/MEBT is hypothesized to promote diabetic wound healing by regulating autophagy. OBJECTIVE: To explore the mechanism of moist exposed MEBT/MEBO promoting diabetic wound repair. MATERIALS AND METHODS: Eighty male Wistar rats were randomly assigned to control (n = 20) and diabetic group induced by intraperitoneal injection of STZ (n = 60), which were further randomly assigned to MEBO, Kangfuxin and model groups (n = 20 each). All rats underwent full-thickness skin resection in the back. Wound healing was dynamically observed and wound tissues were collected at five time points for pathological examination, autophagosome and the expression of PI3K, Akt and mTOR. RESULTS: The healing time in the control group was the shortest, no statistically significant difference was found between the MEBO and the Kangfuxin group (p = 0.76). The morphology of autophagosomes ranged large to small, which was the most obvious in the MEBO group. The mRNA and protein expression of PI3K, Akt and mTOR in each group reached the peak on Day 5, the levels in the MEBO group were the highest (F = 18.43, 19.97, 15.36, p < 0.05). On Day 11, the expression levels in each group began to decline. DISCUSSION AND CONCLUSIONS: In this study, we discussed the molecular mechanism of MEBT/MEBO promoting the repair of diabetic ulcer wounds through autophagy and PI3K-Akt-mTOR signalling pathway, which provides a new way for drug design in the future. Taylor & Francis 2020-01-22 /pmc/articles/PMC7006791/ /pubmed/31967912 http://dx.doi.org/10.1080/13880209.2019.1711430 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zheng, Aitian
Ma, Huadan
Liu, Xianbin
Huang, Qing
Li, Zheng
Wang, Lin
Zeng, Na
Wu, Biaoliang
Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds
title Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds
title_full Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds
title_fullStr Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds
title_full_unstemmed Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds
title_short Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds
title_sort effects of moist exposed burn therapy and ointment (mebt/mebo) on the autophagy mtor signalling pathway in diabetic ulcer wounds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7006791/
https://www.ncbi.nlm.nih.gov/pubmed/31967912
http://dx.doi.org/10.1080/13880209.2019.1711430
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