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Quantitative Imaging of Proteins in Tissue by Stable Isotope Labeled Mimetic Liquid Extraction Surface Analysis Mass Spectrometry
[Image: see text] Absolute quantification of proteins in tissue is important for numerous fields of study. Liquid chromatography–mass spectrometry (LC–MS) methods are the norm but typically involve lengthy sample preparation including tissue homogenization, which results in the loss of information r...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical
Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007001/ https://www.ncbi.nlm.nih.gov/pubmed/31660728 http://dx.doi.org/10.1021/acs.analchem.9b04148 |
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author | Havlikova, Jana Randall, Elizabeth C. Griffiths, Rian L. Swales, John G. Goodwin, Richard J. A. Bunch, Josephine Styles, Iain B. Cooper, Helen J. |
author_facet | Havlikova, Jana Randall, Elizabeth C. Griffiths, Rian L. Swales, John G. Goodwin, Richard J. A. Bunch, Josephine Styles, Iain B. Cooper, Helen J. |
author_sort | Havlikova, Jana |
collection | PubMed |
description | [Image: see text] Absolute quantification of proteins in tissue is important for numerous fields of study. Liquid chromatography–mass spectrometry (LC–MS) methods are the norm but typically involve lengthy sample preparation including tissue homogenization, which results in the loss of information relating to spatial distribution. Here, we propose liquid extraction surface analysis (LESA) mass spectrometry (MS) of stable isotope labeled mimetic tissue models for the spatially resolved quantification of intact ubiquitin in rat and mouse brain tissue. Measured ubiquitin concentrations are in agreement with values found in the literature. Images of rat and mouse brain tissue demonstrate spatial variation in the concentration of ubiquitin and demonstrate the utility of spatially resolved quantitative measurement of proteins in tissue. Although we have focused on ubiquitin, the method has the potential for broader application to the absolute quantitation of any endogenous protein or protein-based drug in tissue. |
format | Online Article Text |
id | pubmed-7007001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American
Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-70070012020-02-10 Quantitative Imaging of Proteins in Tissue by Stable Isotope Labeled Mimetic Liquid Extraction Surface Analysis Mass Spectrometry Havlikova, Jana Randall, Elizabeth C. Griffiths, Rian L. Swales, John G. Goodwin, Richard J. A. Bunch, Josephine Styles, Iain B. Cooper, Helen J. Anal Chem [Image: see text] Absolute quantification of proteins in tissue is important for numerous fields of study. Liquid chromatography–mass spectrometry (LC–MS) methods are the norm but typically involve lengthy sample preparation including tissue homogenization, which results in the loss of information relating to spatial distribution. Here, we propose liquid extraction surface analysis (LESA) mass spectrometry (MS) of stable isotope labeled mimetic tissue models for the spatially resolved quantification of intact ubiquitin in rat and mouse brain tissue. Measured ubiquitin concentrations are in agreement with values found in the literature. Images of rat and mouse brain tissue demonstrate spatial variation in the concentration of ubiquitin and demonstrate the utility of spatially resolved quantitative measurement of proteins in tissue. Although we have focused on ubiquitin, the method has the potential for broader application to the absolute quantitation of any endogenous protein or protein-based drug in tissue. American Chemical Society 2019-10-29 2019-11-19 /pmc/articles/PMC7007001/ /pubmed/31660728 http://dx.doi.org/10.1021/acs.analchem.9b04148 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Havlikova, Jana Randall, Elizabeth C. Griffiths, Rian L. Swales, John G. Goodwin, Richard J. A. Bunch, Josephine Styles, Iain B. Cooper, Helen J. Quantitative Imaging of Proteins in Tissue by Stable Isotope Labeled Mimetic Liquid Extraction Surface Analysis Mass Spectrometry |
title | Quantitative Imaging of Proteins in Tissue by Stable
Isotope Labeled Mimetic Liquid
Extraction Surface Analysis Mass Spectrometry |
title_full | Quantitative Imaging of Proteins in Tissue by Stable
Isotope Labeled Mimetic Liquid
Extraction Surface Analysis Mass Spectrometry |
title_fullStr | Quantitative Imaging of Proteins in Tissue by Stable
Isotope Labeled Mimetic Liquid
Extraction Surface Analysis Mass Spectrometry |
title_full_unstemmed | Quantitative Imaging of Proteins in Tissue by Stable
Isotope Labeled Mimetic Liquid
Extraction Surface Analysis Mass Spectrometry |
title_short | Quantitative Imaging of Proteins in Tissue by Stable
Isotope Labeled Mimetic Liquid
Extraction Surface Analysis Mass Spectrometry |
title_sort | quantitative imaging of proteins in tissue by stable
isotope labeled mimetic liquid
extraction surface analysis mass spectrometry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007001/ https://www.ncbi.nlm.nih.gov/pubmed/31660728 http://dx.doi.org/10.1021/acs.analchem.9b04148 |
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