Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases

[Image: see text] A water-soluble self-assembled supramolecular Fe(II)(4)L(4) tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect th...

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Detalles Bibliográficos
Autores principales: Zhu, Jinbo, Haynes, Cally J. E., Kieffer, Marion, Greenfield, Jake L., Greenhalgh, Ryan D., Nitschke, Jonathan R., Keyser, Ulrich F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007224/
https://www.ncbi.nlm.nih.gov/pubmed/31283214
http://dx.doi.org/10.1021/jacs.9b03566
Descripción
Sumario:[Image: see text] A water-soluble self-assembled supramolecular Fe(II)(4)L(4) tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.

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