Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases

[Image: see text] A water-soluble self-assembled supramolecular Fe(II)(4)L(4) tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect th...

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Autores principales: Zhu, Jinbo, Haynes, Cally J. E., Kieffer, Marion, Greenfield, Jake L., Greenhalgh, Ryan D., Nitschke, Jonathan R., Keyser, Ulrich F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007224/
https://www.ncbi.nlm.nih.gov/pubmed/31283214
http://dx.doi.org/10.1021/jacs.9b03566
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author Zhu, Jinbo
Haynes, Cally J. E.
Kieffer, Marion
Greenfield, Jake L.
Greenhalgh, Ryan D.
Nitschke, Jonathan R.
Keyser, Ulrich F.
author_facet Zhu, Jinbo
Haynes, Cally J. E.
Kieffer, Marion
Greenfield, Jake L.
Greenhalgh, Ryan D.
Nitschke, Jonathan R.
Keyser, Ulrich F.
author_sort Zhu, Jinbo
collection PubMed
description [Image: see text] A water-soluble self-assembled supramolecular Fe(II)(4)L(4) tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.
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spelling pubmed-70072242020-02-10 Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases Zhu, Jinbo Haynes, Cally J. E. Kieffer, Marion Greenfield, Jake L. Greenhalgh, Ryan D. Nitschke, Jonathan R. Keyser, Ulrich F. J Am Chem Soc [Image: see text] A water-soluble self-assembled supramolecular Fe(II)(4)L(4) tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA. American Chemical Society 2019-07-08 2019-07-24 /pmc/articles/PMC7007224/ /pubmed/31283214 http://dx.doi.org/10.1021/jacs.9b03566 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Zhu, Jinbo
Haynes, Cally J. E.
Kieffer, Marion
Greenfield, Jake L.
Greenhalgh, Ryan D.
Nitschke, Jonathan R.
Keyser, Ulrich F.
Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases
title Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases
title_full Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases
title_fullStr Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases
title_full_unstemmed Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases
title_short Fe(II)(4)L(4) Tetrahedron Binds to Nonpaired DNA Bases
title_sort fe(ii)(4)l(4) tetrahedron binds to nonpaired dna bases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007224/
https://www.ncbi.nlm.nih.gov/pubmed/31283214
http://dx.doi.org/10.1021/jacs.9b03566
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