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Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation

Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellular matrix cause a decline in tissue homeostasis, function, and regenerative capacity. Based...

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Autores principales: Etienne, Jessy, Liu, Chao, Skinner, Colin M., Conboy, Michael J., Conboy, Irina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007696/
https://www.ncbi.nlm.nih.gov/pubmed/32033591
http://dx.doi.org/10.1186/s13395-020-0222-1
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author Etienne, Jessy
Liu, Chao
Skinner, Colin M.
Conboy, Michael J.
Conboy, Irina M.
author_facet Etienne, Jessy
Liu, Chao
Skinner, Colin M.
Conboy, Michael J.
Conboy, Irina M.
author_sort Etienne, Jessy
collection PubMed
description Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellular matrix cause a decline in tissue homeostasis, function, and regenerative capacity. Based on the conservation of aging across tissues and taking advantage of the relatively well-characterization of the myofibers and associated SCs, skeletal muscle emerged as an experimental system to study the decline in function and maintenance of old tissues and to explore rejuvenation strategies. In this review, we summarize the approaches for understanding the aging process and for assaying the success of rejuvenation that use skeletal muscle as the experimental system of choice. We further discuss (and exemplify with studies of skeletal muscle) how conflicting results might be due to variations in the techniques of stem cell isolation, differences in the assays of functional rejuvenation, or deciding on the numbers of replicates and experimental cohorts.
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spelling pubmed-70076962020-02-13 Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation Etienne, Jessy Liu, Chao Skinner, Colin M. Conboy, Michael J. Conboy, Irina M. Skelet Muscle Review Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellular matrix cause a decline in tissue homeostasis, function, and regenerative capacity. Based on the conservation of aging across tissues and taking advantage of the relatively well-characterization of the myofibers and associated SCs, skeletal muscle emerged as an experimental system to study the decline in function and maintenance of old tissues and to explore rejuvenation strategies. In this review, we summarize the approaches for understanding the aging process and for assaying the success of rejuvenation that use skeletal muscle as the experimental system of choice. We further discuss (and exemplify with studies of skeletal muscle) how conflicting results might be due to variations in the techniques of stem cell isolation, differences in the assays of functional rejuvenation, or deciding on the numbers of replicates and experimental cohorts. BioMed Central 2020-02-07 /pmc/articles/PMC7007696/ /pubmed/32033591 http://dx.doi.org/10.1186/s13395-020-0222-1 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Etienne, Jessy
Liu, Chao
Skinner, Colin M.
Conboy, Michael J.
Conboy, Irina M.
Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
title Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
title_full Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
title_fullStr Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
title_full_unstemmed Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
title_short Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
title_sort skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007696/
https://www.ncbi.nlm.nih.gov/pubmed/32033591
http://dx.doi.org/10.1186/s13395-020-0222-1
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