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Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation
Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellular matrix cause a decline in tissue homeostasis, function, and regenerative capacity. Based...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007696/ https://www.ncbi.nlm.nih.gov/pubmed/32033591 http://dx.doi.org/10.1186/s13395-020-0222-1 |
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author | Etienne, Jessy Liu, Chao Skinner, Colin M. Conboy, Michael J. Conboy, Irina M. |
author_facet | Etienne, Jessy Liu, Chao Skinner, Colin M. Conboy, Michael J. Conboy, Irina M. |
author_sort | Etienne, Jessy |
collection | PubMed |
description | Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellular matrix cause a decline in tissue homeostasis, function, and regenerative capacity. Based on the conservation of aging across tissues and taking advantage of the relatively well-characterization of the myofibers and associated SCs, skeletal muscle emerged as an experimental system to study the decline in function and maintenance of old tissues and to explore rejuvenation strategies. In this review, we summarize the approaches for understanding the aging process and for assaying the success of rejuvenation that use skeletal muscle as the experimental system of choice. We further discuss (and exemplify with studies of skeletal muscle) how conflicting results might be due to variations in the techniques of stem cell isolation, differences in the assays of functional rejuvenation, or deciding on the numbers of replicates and experimental cohorts. |
format | Online Article Text |
id | pubmed-7007696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-70076962020-02-13 Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation Etienne, Jessy Liu, Chao Skinner, Colin M. Conboy, Michael J. Conboy, Irina M. Skelet Muscle Review Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellular matrix cause a decline in tissue homeostasis, function, and regenerative capacity. Based on the conservation of aging across tissues and taking advantage of the relatively well-characterization of the myofibers and associated SCs, skeletal muscle emerged as an experimental system to study the decline in function and maintenance of old tissues and to explore rejuvenation strategies. In this review, we summarize the approaches for understanding the aging process and for assaying the success of rejuvenation that use skeletal muscle as the experimental system of choice. We further discuss (and exemplify with studies of skeletal muscle) how conflicting results might be due to variations in the techniques of stem cell isolation, differences in the assays of functional rejuvenation, or deciding on the numbers of replicates and experimental cohorts. BioMed Central 2020-02-07 /pmc/articles/PMC7007696/ /pubmed/32033591 http://dx.doi.org/10.1186/s13395-020-0222-1 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Etienne, Jessy Liu, Chao Skinner, Colin M. Conboy, Michael J. Conboy, Irina M. Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation |
title | Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation |
title_full | Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation |
title_fullStr | Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation |
title_full_unstemmed | Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation |
title_short | Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation |
title_sort | skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007696/ https://www.ncbi.nlm.nih.gov/pubmed/32033591 http://dx.doi.org/10.1186/s13395-020-0222-1 |
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