Cargando…

Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice

This study is aimed at investigating the effect of different exercise loads on the reproductive function of obese male mice and the underlying mechanisms. Male mice with high-fat diet-induced obesity were divided into obesity control (OC), obesity moderate-load exercise (OME), and obesity high-load...

Descripción completa

Detalles Bibliográficos
Autores principales: Yi, Xuejie, Tang, Donghui, Cao, Shicheng, Li, Tao, Gao, Haining, Ma, Tie, Yao, Tingting, Li, Jing, Chang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007943/
https://www.ncbi.nlm.nih.gov/pubmed/32082477
http://dx.doi.org/10.1155/2020/3071658
_version_ 1783495389175873536
author Yi, Xuejie
Tang, Donghui
Cao, Shicheng
Li, Tao
Gao, Haining
Ma, Tie
Yao, Tingting
Li, Jing
Chang, Bo
author_facet Yi, Xuejie
Tang, Donghui
Cao, Shicheng
Li, Tao
Gao, Haining
Ma, Tie
Yao, Tingting
Li, Jing
Chang, Bo
author_sort Yi, Xuejie
collection PubMed
description This study is aimed at investigating the effect of different exercise loads on the reproductive function of obese male mice and the underlying mechanisms. Male mice with high-fat diet-induced obesity were divided into obesity control (OC), obesity moderate-load exercise (OME), and obesity high-load exercise (OHE) groups. The OME and OHE groups were subjected to swimming exercise 5 days per week over a duration of 8 weeks, with the exercise load progressively increased to 2 h per day in the OME group and 2 h twice per day in the OHE group. In the OC group mice without exercise regimen, we observed a decrease in mRNA expression of antioxidant enzymes, increase in free radical products, upregulation of mRNA and protein expression of nuclear factor-κB and proinflammatory cytokines, inhibition of mRNA and protein expression of testosterone synthases, decrease in the serum testosterone level and sperm quality, and increase in sperm apoptosis. Although both moderate-load exercise and high-load exercise reduced body fat, only moderate-load exercise effectively alleviated obesity-induced oxidative stress, downregulated the expression of nuclear factor-κB and proinflammatory cytokines, and reversed the decrease in mRNA and protein expression of testosterone synthases, serum testosterone level, and sperm quality. These changes were not observed in the OHE group mice. Obesity-induced testicular oxidative stress and inflammatory response decreased testosterone synthesis and sperm quality. Moderate-load exercise alleviated the negative effect of obesity on male reproductive function by decreasing testicular oxidative stress and inflammatory responses. Although high-load exercise effectively reduced body fat, its effects on alleviating oxidative stress and improving male reproductive function were limited.
format Online
Article
Text
id pubmed-7007943
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-70079432020-02-20 Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice Yi, Xuejie Tang, Donghui Cao, Shicheng Li, Tao Gao, Haining Ma, Tie Yao, Tingting Li, Jing Chang, Bo Oxid Med Cell Longev Research Article This study is aimed at investigating the effect of different exercise loads on the reproductive function of obese male mice and the underlying mechanisms. Male mice with high-fat diet-induced obesity were divided into obesity control (OC), obesity moderate-load exercise (OME), and obesity high-load exercise (OHE) groups. The OME and OHE groups were subjected to swimming exercise 5 days per week over a duration of 8 weeks, with the exercise load progressively increased to 2 h per day in the OME group and 2 h twice per day in the OHE group. In the OC group mice without exercise regimen, we observed a decrease in mRNA expression of antioxidant enzymes, increase in free radical products, upregulation of mRNA and protein expression of nuclear factor-κB and proinflammatory cytokines, inhibition of mRNA and protein expression of testosterone synthases, decrease in the serum testosterone level and sperm quality, and increase in sperm apoptosis. Although both moderate-load exercise and high-load exercise reduced body fat, only moderate-load exercise effectively alleviated obesity-induced oxidative stress, downregulated the expression of nuclear factor-κB and proinflammatory cytokines, and reversed the decrease in mRNA and protein expression of testosterone synthases, serum testosterone level, and sperm quality. These changes were not observed in the OHE group mice. Obesity-induced testicular oxidative stress and inflammatory response decreased testosterone synthesis and sperm quality. Moderate-load exercise alleviated the negative effect of obesity on male reproductive function by decreasing testicular oxidative stress and inflammatory responses. Although high-load exercise effectively reduced body fat, its effects on alleviating oxidative stress and improving male reproductive function were limited. Hindawi 2020-01-27 /pmc/articles/PMC7007943/ /pubmed/32082477 http://dx.doi.org/10.1155/2020/3071658 Text en Copyright © 2020 Xuejie Yi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yi, Xuejie
Tang, Donghui
Cao, Shicheng
Li, Tao
Gao, Haining
Ma, Tie
Yao, Tingting
Li, Jing
Chang, Bo
Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice
title Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice
title_full Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice
title_fullStr Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice
title_full_unstemmed Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice
title_short Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice
title_sort effect of different exercise loads on testicular oxidative stress and reproductive function in obese male mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007943/
https://www.ncbi.nlm.nih.gov/pubmed/32082477
http://dx.doi.org/10.1155/2020/3071658
work_keys_str_mv AT yixuejie effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT tangdonghui effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT caoshicheng effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT litao effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT gaohaining effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT matie effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT yaotingting effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT lijing effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice
AT changbo effectofdifferentexerciseloadsontesticularoxidativestressandreproductivefunctioninobesemalemice