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Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice
This study is aimed at investigating the effect of different exercise loads on the reproductive function of obese male mice and the underlying mechanisms. Male mice with high-fat diet-induced obesity were divided into obesity control (OC), obesity moderate-load exercise (OME), and obesity high-load...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007943/ https://www.ncbi.nlm.nih.gov/pubmed/32082477 http://dx.doi.org/10.1155/2020/3071658 |
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author | Yi, Xuejie Tang, Donghui Cao, Shicheng Li, Tao Gao, Haining Ma, Tie Yao, Tingting Li, Jing Chang, Bo |
author_facet | Yi, Xuejie Tang, Donghui Cao, Shicheng Li, Tao Gao, Haining Ma, Tie Yao, Tingting Li, Jing Chang, Bo |
author_sort | Yi, Xuejie |
collection | PubMed |
description | This study is aimed at investigating the effect of different exercise loads on the reproductive function of obese male mice and the underlying mechanisms. Male mice with high-fat diet-induced obesity were divided into obesity control (OC), obesity moderate-load exercise (OME), and obesity high-load exercise (OHE) groups. The OME and OHE groups were subjected to swimming exercise 5 days per week over a duration of 8 weeks, with the exercise load progressively increased to 2 h per day in the OME group and 2 h twice per day in the OHE group. In the OC group mice without exercise regimen, we observed a decrease in mRNA expression of antioxidant enzymes, increase in free radical products, upregulation of mRNA and protein expression of nuclear factor-κB and proinflammatory cytokines, inhibition of mRNA and protein expression of testosterone synthases, decrease in the serum testosterone level and sperm quality, and increase in sperm apoptosis. Although both moderate-load exercise and high-load exercise reduced body fat, only moderate-load exercise effectively alleviated obesity-induced oxidative stress, downregulated the expression of nuclear factor-κB and proinflammatory cytokines, and reversed the decrease in mRNA and protein expression of testosterone synthases, serum testosterone level, and sperm quality. These changes were not observed in the OHE group mice. Obesity-induced testicular oxidative stress and inflammatory response decreased testosterone synthesis and sperm quality. Moderate-load exercise alleviated the negative effect of obesity on male reproductive function by decreasing testicular oxidative stress and inflammatory responses. Although high-load exercise effectively reduced body fat, its effects on alleviating oxidative stress and improving male reproductive function were limited. |
format | Online Article Text |
id | pubmed-7007943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-70079432020-02-20 Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice Yi, Xuejie Tang, Donghui Cao, Shicheng Li, Tao Gao, Haining Ma, Tie Yao, Tingting Li, Jing Chang, Bo Oxid Med Cell Longev Research Article This study is aimed at investigating the effect of different exercise loads on the reproductive function of obese male mice and the underlying mechanisms. Male mice with high-fat diet-induced obesity were divided into obesity control (OC), obesity moderate-load exercise (OME), and obesity high-load exercise (OHE) groups. The OME and OHE groups were subjected to swimming exercise 5 days per week over a duration of 8 weeks, with the exercise load progressively increased to 2 h per day in the OME group and 2 h twice per day in the OHE group. In the OC group mice without exercise regimen, we observed a decrease in mRNA expression of antioxidant enzymes, increase in free radical products, upregulation of mRNA and protein expression of nuclear factor-κB and proinflammatory cytokines, inhibition of mRNA and protein expression of testosterone synthases, decrease in the serum testosterone level and sperm quality, and increase in sperm apoptosis. Although both moderate-load exercise and high-load exercise reduced body fat, only moderate-load exercise effectively alleviated obesity-induced oxidative stress, downregulated the expression of nuclear factor-κB and proinflammatory cytokines, and reversed the decrease in mRNA and protein expression of testosterone synthases, serum testosterone level, and sperm quality. These changes were not observed in the OHE group mice. Obesity-induced testicular oxidative stress and inflammatory response decreased testosterone synthesis and sperm quality. Moderate-load exercise alleviated the negative effect of obesity on male reproductive function by decreasing testicular oxidative stress and inflammatory responses. Although high-load exercise effectively reduced body fat, its effects on alleviating oxidative stress and improving male reproductive function were limited. Hindawi 2020-01-27 /pmc/articles/PMC7007943/ /pubmed/32082477 http://dx.doi.org/10.1155/2020/3071658 Text en Copyright © 2020 Xuejie Yi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yi, Xuejie Tang, Donghui Cao, Shicheng Li, Tao Gao, Haining Ma, Tie Yao, Tingting Li, Jing Chang, Bo Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice |
title | Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice |
title_full | Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice |
title_fullStr | Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice |
title_full_unstemmed | Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice |
title_short | Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice |
title_sort | effect of different exercise loads on testicular oxidative stress and reproductive function in obese male mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007943/ https://www.ncbi.nlm.nih.gov/pubmed/32082477 http://dx.doi.org/10.1155/2020/3071658 |
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