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Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats

Intratracheal instillation is the introduction of a substance directly into the trachea. Intratracheal instillation has been used to investigate the lung toxicity of several chemicals and requires the suspension or dissolution of test material in a vehicle for even dispersal throughout the lung. Imp...

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Autores principales: Numano, Takamasa, Morioka, Mai, Higuchi, Hitomi, Uda, Kazunari, Sugiyama, Taiki, Hagiwara, Teruaki, Doi, Yuko, Imai, Norio, Kawabe, Mayumi, Mera, Yukinori, Tamano, Seiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Toxicologic Pathology 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008201/
https://www.ncbi.nlm.nih.gov/pubmed/32051660
http://dx.doi.org/10.1293/tox.2019-0060
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author Numano, Takamasa
Morioka, Mai
Higuchi, Hitomi
Uda, Kazunari
Sugiyama, Taiki
Hagiwara, Teruaki
Doi, Yuko
Imai, Norio
Kawabe, Mayumi
Mera, Yukinori
Tamano, Seiko
author_facet Numano, Takamasa
Morioka, Mai
Higuchi, Hitomi
Uda, Kazunari
Sugiyama, Taiki
Hagiwara, Teruaki
Doi, Yuko
Imai, Norio
Kawabe, Mayumi
Mera, Yukinori
Tamano, Seiko
author_sort Numano, Takamasa
collection PubMed
description Intratracheal instillation is the introduction of a substance directly into the trachea. Intratracheal instillation has been used to investigate the lung toxicity of several chemicals and requires the suspension or dissolution of test material in a vehicle for even dispersal throughout the lung. Importantly, the toxicities of vehicles used in intratracheal instillation studies are generally considered to be insignificant. Hence, evaluating the influence of different vehicles on the lung due to intratracheal instillation is crucial. We examined the toxic effects of pure water, saline, phosphate buffered saline (PBS), 0.5% Kolliphor(®) P188 (KP188), 0.1% Tween 20 in saline, and 1.0% BSA in PBS. These vehicles were administered to male Crl:CD(SD) rats by a single intratracheal instillation. On day 3, broncho-alveolar lavage fluid (BALF) from the right lung was collected and processed for cell counting and biochemical analysis, while the left lung was used for histopathological examination. Accumulation of alveolar macrophages was observed in all vehicle-treated groups but was minimal in the group administered saline, somewhat higher in the groups administered pure water, PBS, 0.1% Tween 20, and 1% BSA, and notably higher in the group administered 0.5% KP188. The results from BALF analysis indicated that intratracheal instillation of 0.5% KP188 also induced alveolar damage. Additionally, administering pure water did not appear to cause tissue damage. Eosinophil infiltration in the interstitial regions was histopathologically observed. Altogether, the results of this study are helpful for the selection of appropriate vehicles for use in intratracheal instillation studies.
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spelling pubmed-70082012020-02-12 Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats Numano, Takamasa Morioka, Mai Higuchi, Hitomi Uda, Kazunari Sugiyama, Taiki Hagiwara, Teruaki Doi, Yuko Imai, Norio Kawabe, Mayumi Mera, Yukinori Tamano, Seiko J Toxicol Pathol Original Article Intratracheal instillation is the introduction of a substance directly into the trachea. Intratracheal instillation has been used to investigate the lung toxicity of several chemicals and requires the suspension or dissolution of test material in a vehicle for even dispersal throughout the lung. Importantly, the toxicities of vehicles used in intratracheal instillation studies are generally considered to be insignificant. Hence, evaluating the influence of different vehicles on the lung due to intratracheal instillation is crucial. We examined the toxic effects of pure water, saline, phosphate buffered saline (PBS), 0.5% Kolliphor(®) P188 (KP188), 0.1% Tween 20 in saline, and 1.0% BSA in PBS. These vehicles were administered to male Crl:CD(SD) rats by a single intratracheal instillation. On day 3, broncho-alveolar lavage fluid (BALF) from the right lung was collected and processed for cell counting and biochemical analysis, while the left lung was used for histopathological examination. Accumulation of alveolar macrophages was observed in all vehicle-treated groups but was minimal in the group administered saline, somewhat higher in the groups administered pure water, PBS, 0.1% Tween 20, and 1% BSA, and notably higher in the group administered 0.5% KP188. The results from BALF analysis indicated that intratracheal instillation of 0.5% KP188 also induced alveolar damage. Additionally, administering pure water did not appear to cause tissue damage. Eosinophil infiltration in the interstitial regions was histopathologically observed. Altogether, the results of this study are helpful for the selection of appropriate vehicles for use in intratracheal instillation studies. Japanese Society of Toxicologic Pathology 2019-09-28 2020-01 /pmc/articles/PMC7008201/ /pubmed/32051660 http://dx.doi.org/10.1293/tox.2019-0060 Text en ©2020 The Japanese Society of Toxicologic Pathology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Numano, Takamasa
Morioka, Mai
Higuchi, Hitomi
Uda, Kazunari
Sugiyama, Taiki
Hagiwara, Teruaki
Doi, Yuko
Imai, Norio
Kawabe, Mayumi
Mera, Yukinori
Tamano, Seiko
Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats
title Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats
title_full Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats
title_fullStr Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats
title_full_unstemmed Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats
title_short Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats
title_sort effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of crl:cd(sd) rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008201/
https://www.ncbi.nlm.nih.gov/pubmed/32051660
http://dx.doi.org/10.1293/tox.2019-0060
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