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OpenSFDI: an open-source guide for constructing a spatial frequency domain imaging system

Significance: Spatial frequency domain imaging (SFDI) is a diffuse optical measurement technique that can quantify tissue optical absorption ([Formula: see text]) and reduced scattering ([Formula: see text]) on a pixel-by-pixel basis. Measurements of [Formula: see text] at different wavelengths enab...

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Detalles Bibliográficos
Autores principales: Applegate, Matthew B., Karrobi, Kavon, Angelo Jr., Joseph P., Austin, Wyatt, Tabassum, Syeda M., Aguénounon, Enagnon, Tilbury, Karissa, Saager, Rolf B., Gioux, Sylvain, Roblyer, Darren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008504/
https://www.ncbi.nlm.nih.gov/pubmed/31925946
http://dx.doi.org/10.1117/1.JBO.25.1.016002
Descripción
Sumario:Significance: Spatial frequency domain imaging (SFDI) is a diffuse optical measurement technique that can quantify tissue optical absorption ([Formula: see text]) and reduced scattering ([Formula: see text]) on a pixel-by-pixel basis. Measurements of [Formula: see text] at different wavelengths enable the extraction of molar concentrations of tissue chromophores over a wide field, providing a noncontact and label-free means to assess tissue viability, oxygenation, microarchitecture, and molecular content. We present here openSFDI: an open-source guide for building a low-cost, small-footprint, three-wavelength SFDI system capable of quantifying [Formula: see text] and [Formula: see text] as well as oxyhemoglobin and deoxyhemoglobin concentrations in biological tissue. The companion website provides a complete parts list along with detailed instructions for assembling the openSFDI system. Aim: We describe the design of openSFDI and report on the accuracy and precision of optical property extractions for three different systems fabricated according to the instructions on the openSFDI website. Approach: Accuracy was assessed by measuring nine tissue-simulating optical phantoms with a physiologically relevant range of [Formula: see text] and [Formula: see text] with the openSFDI systems and a commercial SFDI device. Precision was assessed by repeatedly measuring the same phantom over 1 h. Results: The openSFDI systems had an error of [Formula: see text] in [Formula: see text] and [Formula: see text] in [Formula: see text] , compared to a commercial SFDI system. Bland–Altman analysis revealed the limits of agreement between the two systems to be [Formula: see text] for [Formula: see text] and [Formula: see text] to [Formula: see text] for [Formula: see text]. The openSFDI system had low drift with an average standard deviation of [Formula: see text] and [Formula: see text] in [Formula: see text] and [Formula: see text] , respectively. Conclusion: The openSFDI provides a customizable hardware platform for research groups seeking to utilize SFDI for quantitative diffuse optical imaging.