Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient

Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emissio...

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Detalles Bibliográficos
Autores principales: Lee, Han-Kyu, Morin, Peter, Xia, Weiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008971/
https://www.ncbi.nlm.nih.gov/pubmed/27345971
http://dx.doi.org/10.1016/j.scr.2015.12.050
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author Lee, Han-Kyu
Morin, Peter
Xia, Weiming
author_facet Lee, Han-Kyu
Morin, Peter
Xia, Weiming
author_sort Lee, Han-Kyu
collection PubMed
description Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emission tomography scan is consistent with a diagnosis of Alzheimer's disease. Integration-free CytoTune-iPS Sendai Reprogramming factors which include Sendai virus particles of the four Yamanaka factors Oct4, Sox2, Klf4, and c-Myc were introduced to PBMC to convert them to iPSCs without retention of virus. Three germ layer differentiation was induced to demonstrate the pluripotency of these iPSCs.
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spelling pubmed-70089712020-02-10 Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient Lee, Han-Kyu Morin, Peter Xia, Weiming Stem Cell Res Article Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emission tomography scan is consistent with a diagnosis of Alzheimer's disease. Integration-free CytoTune-iPS Sendai Reprogramming factors which include Sendai virus particles of the four Yamanaka factors Oct4, Sox2, Klf4, and c-Myc were introduced to PBMC to convert them to iPSCs without retention of virus. Three germ layer differentiation was induced to demonstrate the pluripotency of these iPSCs. 2016-01-15 2016-03 /pmc/articles/PMC7008971/ /pubmed/27345971 http://dx.doi.org/10.1016/j.scr.2015.12.050 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lee, Han-Kyu
Morin, Peter
Xia, Weiming
Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient
title Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient
title_full Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient
title_fullStr Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient
title_full_unstemmed Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient
title_short Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient
title_sort peripheral blood mononuclear cell-converted induced pluripotent stem cells (ipscs) from an early onset alzheimer's patient
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008971/
https://www.ncbi.nlm.nih.gov/pubmed/27345971
http://dx.doi.org/10.1016/j.scr.2015.12.050
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AT xiaweiming peripheralbloodmononuclearcellconvertedinducedpluripotentstemcellsipscsfromanearlyonsetalzheimerspatient

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