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Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles

Sperm preparation in IVF cycles using density gradient centrifugation (DGC) in combination with swim-up (SU) has been widely adopted in reproductive centres worldwide. It is a fact that the sperm recovery rate following one DGC from poor semen samples (showing liquefaction defects/containing too man...

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Autores principales: Dai, Xiuliang, Wang, Yufeng, Cao, Fang, Yu, Chunmei, Gao, Tingting, Xia, Xiyang, Wu, Jun, Chen, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010666/
https://www.ncbi.nlm.nih.gov/pubmed/32042061
http://dx.doi.org/10.1038/s41598-020-59347-y
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author Dai, Xiuliang
Wang, Yufeng
Cao, Fang
Yu, Chunmei
Gao, Tingting
Xia, Xiyang
Wu, Jun
Chen, Li
author_facet Dai, Xiuliang
Wang, Yufeng
Cao, Fang
Yu, Chunmei
Gao, Tingting
Xia, Xiyang
Wu, Jun
Chen, Li
author_sort Dai, Xiuliang
collection PubMed
description Sperm preparation in IVF cycles using density gradient centrifugation (DGC) in combination with swim-up (SU) has been widely adopted in reproductive centres worldwide. It is a fact that the sperm recovery rate following one DGC from poor semen samples (showing liquefaction defects/containing too many unresolvable clots or rare sperm) is relatively low. Our results showed that double DGC (DDGC) is effective at increasing the sperm recovery rate from poor semen samples. However, DDGC may increase the mechanical stress of sperm, thereby potentially impairing embryo development. Therefore, it is necessary to evaluate the safety of using sperm prepared by DDGC/SU for IVF cycles. In this study, we retrospectively analysed the data generated from a total of 529 IVF cycles (from June 2017 to June 2018), and these IVF cycles contributed 622 transfer cycles (from June 2017 to December 2018) in Changzhou Maternal and Child Health Care Hospital. Of them, 306 IVF cycles and the related 355 transfer cycles (normal semen samples prepared by DGC/SU) were set as the normal group, while 223 IVF cycles and the related 267 transfer cycles (poor semen prepared by DDGC/SU) were set as the observation group. The main outcome measures, including the normal fertilization rate, top D3 embryo formation rate, blastocyte formation rate, clinical pregnancy rate and live birth rate, birth weight and duration of pregnancy, were compared between the two groups. Compared to semen in the DGC/SU group, semen in the DDGC/SU group showed increased levels of the DNA fragmentation index (DFI) and reduced sperm concentration, percentage of progressive motility (PR) sperm, and percentage of normal morphology sperm. The indicators reflecting in vitro embryo development and clinical outcomes were similar in the DGC/SU group and DDGC/SU group, including the normal fertilization rate, top D3 embryo formation rate, blastocyte formation rate, pregnancy rate, implantation rate, spontaneous abortion rate, live birth rate, birth weight and duration of pregnancy. Furthermore, we found that the 1PN zygote formation rate was significantly lower in the DDGC/SU group than that in the DGC/SU group. We concluded that oocytes fertilized by sperm from poor semen samples separated by DDGC/SU achieved the same outcomes as oocytes fertilized by sperm from normal semen separated by DGC/SU, suggesting that DDGC/SU is an effective and safe method of sperm enrichment for poor semen samples in IVF. The main contribution of the present study is the verification of the effectiveness of DDGC/SU in improving sperm recovery from poor semen samples and the safety of using sperm prepared by DDGC/SU for IVF.
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spelling pubmed-70106662020-02-21 Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles Dai, Xiuliang Wang, Yufeng Cao, Fang Yu, Chunmei Gao, Tingting Xia, Xiyang Wu, Jun Chen, Li Sci Rep Article Sperm preparation in IVF cycles using density gradient centrifugation (DGC) in combination with swim-up (SU) has been widely adopted in reproductive centres worldwide. It is a fact that the sperm recovery rate following one DGC from poor semen samples (showing liquefaction defects/containing too many unresolvable clots or rare sperm) is relatively low. Our results showed that double DGC (DDGC) is effective at increasing the sperm recovery rate from poor semen samples. However, DDGC may increase the mechanical stress of sperm, thereby potentially impairing embryo development. Therefore, it is necessary to evaluate the safety of using sperm prepared by DDGC/SU for IVF cycles. In this study, we retrospectively analysed the data generated from a total of 529 IVF cycles (from June 2017 to June 2018), and these IVF cycles contributed 622 transfer cycles (from June 2017 to December 2018) in Changzhou Maternal and Child Health Care Hospital. Of them, 306 IVF cycles and the related 355 transfer cycles (normal semen samples prepared by DGC/SU) were set as the normal group, while 223 IVF cycles and the related 267 transfer cycles (poor semen prepared by DDGC/SU) were set as the observation group. The main outcome measures, including the normal fertilization rate, top D3 embryo formation rate, blastocyte formation rate, clinical pregnancy rate and live birth rate, birth weight and duration of pregnancy, were compared between the two groups. Compared to semen in the DGC/SU group, semen in the DDGC/SU group showed increased levels of the DNA fragmentation index (DFI) and reduced sperm concentration, percentage of progressive motility (PR) sperm, and percentage of normal morphology sperm. The indicators reflecting in vitro embryo development and clinical outcomes were similar in the DGC/SU group and DDGC/SU group, including the normal fertilization rate, top D3 embryo formation rate, blastocyte formation rate, pregnancy rate, implantation rate, spontaneous abortion rate, live birth rate, birth weight and duration of pregnancy. Furthermore, we found that the 1PN zygote formation rate was significantly lower in the DDGC/SU group than that in the DGC/SU group. We concluded that oocytes fertilized by sperm from poor semen samples separated by DDGC/SU achieved the same outcomes as oocytes fertilized by sperm from normal semen separated by DGC/SU, suggesting that DDGC/SU is an effective and safe method of sperm enrichment for poor semen samples in IVF. The main contribution of the present study is the verification of the effectiveness of DDGC/SU in improving sperm recovery from poor semen samples and the safety of using sperm prepared by DDGC/SU for IVF. Nature Publishing Group UK 2020-02-10 /pmc/articles/PMC7010666/ /pubmed/32042061 http://dx.doi.org/10.1038/s41598-020-59347-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dai, Xiuliang
Wang, Yufeng
Cao, Fang
Yu, Chunmei
Gao, Tingting
Xia, Xiyang
Wu, Jun
Chen, Li
Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles
title Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles
title_full Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles
title_fullStr Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles
title_full_unstemmed Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles
title_short Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles
title_sort sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for ivf cycles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010666/
https://www.ncbi.nlm.nih.gov/pubmed/32042061
http://dx.doi.org/10.1038/s41598-020-59347-y
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