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Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides

Understanding the effect of pesticides on the survival of honeybee colonies is important because these pollinators are reportedly declining globally. In the present study, we examined the changes in the head proteome of nurse honeybees exposed to individual and combined pesticides (the fungicide pyr...

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Autores principales: Zaluski, Rodrigo, Bittarello, Alis Correia, Vieira, José Cavalcante Souza, Braga, Camila Pereira, Padilha, Pedro de Magalhaes, Fernandes, Mileni da Silva, Bovi, Thaís de Souza, Orsi, Ricardo de Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010795/
https://www.ncbi.nlm.nih.gov/pubmed/32042077
http://dx.doi.org/10.1038/s41598-020-59070-8
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author Zaluski, Rodrigo
Bittarello, Alis Correia
Vieira, José Cavalcante Souza
Braga, Camila Pereira
Padilha, Pedro de Magalhaes
Fernandes, Mileni da Silva
Bovi, Thaís de Souza
Orsi, Ricardo de Oliveira
author_facet Zaluski, Rodrigo
Bittarello, Alis Correia
Vieira, José Cavalcante Souza
Braga, Camila Pereira
Padilha, Pedro de Magalhaes
Fernandes, Mileni da Silva
Bovi, Thaís de Souza
Orsi, Ricardo de Oliveira
author_sort Zaluski, Rodrigo
collection PubMed
description Understanding the effect of pesticides on the survival of honeybee colonies is important because these pollinators are reportedly declining globally. In the present study, we examined the changes in the head proteome of nurse honeybees exposed to individual and combined pesticides (the fungicide pyraclostrobin and the insecticide fipronil) at field-relevant doses (850 and 2.5 ppb, respectively). The head proteomes of bees exposed to pesticides were compared with those of bees that were not exposed, and proteins with differences in expression were identified by mass spectrometry. The exposure of nurse bees to pesticides reduced the expression of four of the major royal jelly proteins (MRJP1, MRJP2, MRJP4, and MRJP5) and also several proteins associated with carbohydrate metabolism and energy synthesis, the antioxidant system, detoxification, biosynthesis, amino acid metabolism, transcription and translation, protein folding and binding, olfaction, and learning and memory. Overall, when pyraclostrobin and fipronil were combined, the changes in protein expression were exacerbated. Our results demonstrate that vital proteins and metabolic processes are impaired in nurse honeybees exposed to pesticides in doses close to those experienced by these insects in the field, increasing their susceptibility to stressors and affecting the nutrition and maintenance of both managed and natural colonies.
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spelling pubmed-70107952020-02-21 Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides Zaluski, Rodrigo Bittarello, Alis Correia Vieira, José Cavalcante Souza Braga, Camila Pereira Padilha, Pedro de Magalhaes Fernandes, Mileni da Silva Bovi, Thaís de Souza Orsi, Ricardo de Oliveira Sci Rep Article Understanding the effect of pesticides on the survival of honeybee colonies is important because these pollinators are reportedly declining globally. In the present study, we examined the changes in the head proteome of nurse honeybees exposed to individual and combined pesticides (the fungicide pyraclostrobin and the insecticide fipronil) at field-relevant doses (850 and 2.5 ppb, respectively). The head proteomes of bees exposed to pesticides were compared with those of bees that were not exposed, and proteins with differences in expression were identified by mass spectrometry. The exposure of nurse bees to pesticides reduced the expression of four of the major royal jelly proteins (MRJP1, MRJP2, MRJP4, and MRJP5) and also several proteins associated with carbohydrate metabolism and energy synthesis, the antioxidant system, detoxification, biosynthesis, amino acid metabolism, transcription and translation, protein folding and binding, olfaction, and learning and memory. Overall, when pyraclostrobin and fipronil were combined, the changes in protein expression were exacerbated. Our results demonstrate that vital proteins and metabolic processes are impaired in nurse honeybees exposed to pesticides in doses close to those experienced by these insects in the field, increasing their susceptibility to stressors and affecting the nutrition and maintenance of both managed and natural colonies. Nature Publishing Group UK 2020-02-10 /pmc/articles/PMC7010795/ /pubmed/32042077 http://dx.doi.org/10.1038/s41598-020-59070-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zaluski, Rodrigo
Bittarello, Alis Correia
Vieira, José Cavalcante Souza
Braga, Camila Pereira
Padilha, Pedro de Magalhaes
Fernandes, Mileni da Silva
Bovi, Thaís de Souza
Orsi, Ricardo de Oliveira
Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides
title Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides
title_full Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides
title_fullStr Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides
title_full_unstemmed Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides
title_short Modification of the head proteome of nurse honeybees (Apis mellifera) exposed to field-relevant doses of pesticides
title_sort modification of the head proteome of nurse honeybees (apis mellifera) exposed to field-relevant doses of pesticides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010795/
https://www.ncbi.nlm.nih.gov/pubmed/32042077
http://dx.doi.org/10.1038/s41598-020-59070-8
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