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Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase
This article reports experimental data related to the research article entitled “Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase” (R.R. Garafutdinov, A.R. Sakhabutdinova, M.S. Kupryushkin, D.V. Pyshnyi, 2020) [1]. Here...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011048/ https://www.ncbi.nlm.nih.gov/pubmed/32071971 http://dx.doi.org/10.1016/j.dib.2020.105188 |
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author | Garafutdinov, Ravil R. Sakhabutdinova, Assol R. Kupryushkin, Maxim S. Pyshnyi, Dmitrii V. |
author_facet | Garafutdinov, Ravil R. Sakhabutdinova, Assol R. Kupryushkin, Maxim S. Pyshnyi, Dmitrii V. |
author_sort | Garafutdinov, Ravil R. |
collection | PubMed |
description | This article reports experimental data related to the research article entitled “Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase” (R.R. Garafutdinov, A.R. Sakhabutdinova, M.S. Kupryushkin, D.V. Pyshnyi, 2020) [1]. Here, multimerization efficiency in terms of Tt (time-to-threshold) values obtained for artificial DNA templates with the different nucleotide sequences during isothermal amplification with Bst exo- DNA polymerase is given. Data on the influence of phosphoryl guanidine primers (PGO) on multimerization for the LTc template which has shown high efficiency of multimerization are presented as well. |
format | Online Article Text |
id | pubmed-7011048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70110482020-02-18 Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase Garafutdinov, Ravil R. Sakhabutdinova, Assol R. Kupryushkin, Maxim S. Pyshnyi, Dmitrii V. Data Brief Biochemistry, Genetics and Molecular Biology This article reports experimental data related to the research article entitled “Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase” (R.R. Garafutdinov, A.R. Sakhabutdinova, M.S. Kupryushkin, D.V. Pyshnyi, 2020) [1]. Here, multimerization efficiency in terms of Tt (time-to-threshold) values obtained for artificial DNA templates with the different nucleotide sequences during isothermal amplification with Bst exo- DNA polymerase is given. Data on the influence of phosphoryl guanidine primers (PGO) on multimerization for the LTc template which has shown high efficiency of multimerization are presented as well. Elsevier 2020-01-25 /pmc/articles/PMC7011048/ /pubmed/32071971 http://dx.doi.org/10.1016/j.dib.2020.105188 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Garafutdinov, Ravil R. Sakhabutdinova, Assol R. Kupryushkin, Maxim S. Pyshnyi, Dmitrii V. Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase |
title | Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase |
title_full | Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase |
title_fullStr | Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase |
title_full_unstemmed | Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase |
title_short | Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase |
title_sort | data on multimerization efficiency for short linear dna templates and phosphoryl guanidine primers during isothermal amplification with bst exo- dna polymerase |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011048/ https://www.ncbi.nlm.nih.gov/pubmed/32071971 http://dx.doi.org/10.1016/j.dib.2020.105188 |
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