Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide

[Image: see text] We describe here the development of a photoreleasable version of a protein phosphatase-1 (PP1)-disrupting peptide (PDP-Nal) that triggers protein phosphatase-1 activity. PDP-Nal is a 23 mer that binds to PP1 through several interactions. It was photocaged on a tyrosine residue, whi...

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Autores principales: Trebacz, Malgorzata, Wang, Yansong, Makotta, Leslie, Henschke, Lars, Köhn, Maja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011174/
https://www.ncbi.nlm.nih.gov/pubmed/31841001
http://dx.doi.org/10.1021/acs.joc.9b02548
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author Trebacz, Malgorzata
Wang, Yansong
Makotta, Leslie
Henschke, Lars
Köhn, Maja
author_facet Trebacz, Malgorzata
Wang, Yansong
Makotta, Leslie
Henschke, Lars
Köhn, Maja
author_sort Trebacz, Malgorzata
collection PubMed
description [Image: see text] We describe here the development of a photoreleasable version of a protein phosphatase-1 (PP1)-disrupting peptide (PDP-Nal) that triggers protein phosphatase-1 activity. PDP-Nal is a 23 mer that binds to PP1 through several interactions. It was photocaged on a tyrosine residue, which required the exchange of phenylalanine in PDP-Nal to tyrosine in order to disrupt the most important binding interface. This PDP-caged can be light-controlled in live cells.
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spelling pubmed-70111742020-02-12 Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide Trebacz, Malgorzata Wang, Yansong Makotta, Leslie Henschke, Lars Köhn, Maja J Org Chem [Image: see text] We describe here the development of a photoreleasable version of a protein phosphatase-1 (PP1)-disrupting peptide (PDP-Nal) that triggers protein phosphatase-1 activity. PDP-Nal is a 23 mer that binds to PP1 through several interactions. It was photocaged on a tyrosine residue, which required the exchange of phenylalanine in PDP-Nal to tyrosine in order to disrupt the most important binding interface. This PDP-caged can be light-controlled in live cells. American Chemical Society 2019-12-16 2020-02-07 /pmc/articles/PMC7011174/ /pubmed/31841001 http://dx.doi.org/10.1021/acs.joc.9b02548 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Trebacz, Malgorzata
Wang, Yansong
Makotta, Leslie
Henschke, Lars
Köhn, Maja
Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide
title Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide
title_full Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide
title_fullStr Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide
title_full_unstemmed Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide
title_short Development of a Photoactivatable Protein Phosphatase-1-Disrupting Peptide
title_sort development of a photoactivatable protein phosphatase-1-disrupting peptide
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011174/
https://www.ncbi.nlm.nih.gov/pubmed/31841001
http://dx.doi.org/10.1021/acs.joc.9b02548
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