Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein

Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This...

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Autores principales: Yue, Yu‐Xin, Kong, Yong, Yang, Fan, Zheng, Zhe, Hu, Xin‐Yue, Guo, Dong‐Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011187/
https://www.ncbi.nlm.nih.gov/pubmed/32071829
http://dx.doi.org/10.1002/open.201900316
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author Yue, Yu‐Xin
Kong, Yong
Yang, Fan
Zheng, Zhe
Hu, Xin‐Yue
Guo, Dong‐Sheng
author_facet Yue, Yu‐Xin
Kong, Yong
Yang, Fan
Zheng, Zhe
Hu, Xin‐Yue
Guo, Dong‐Sheng
author_sort Yue, Yu‐Xin
collection PubMed
description Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This supramolecular tandem assay system offers an opportunity to monitor the activity of ALP in a label‐free, continuous, and real‐time manner. More importantly, a calibration curve can be given for selective and quantitative detection of pyridoxal‐5′‐phosphate (biomarker for several diseases).
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spelling pubmed-70111872020-02-18 Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein Yue, Yu‐Xin Kong, Yong Yang, Fan Zheng, Zhe Hu, Xin‐Yue Guo, Dong‐Sheng ChemistryOpen Communications Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This supramolecular tandem assay system offers an opportunity to monitor the activity of ALP in a label‐free, continuous, and real‐time manner. More importantly, a calibration curve can be given for selective and quantitative detection of pyridoxal‐5′‐phosphate (biomarker for several diseases). John Wiley and Sons Inc. 2019-12-13 /pmc/articles/PMC7011187/ /pubmed/32071829 http://dx.doi.org/10.1002/open.201900316 Text en © 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Communications
Yue, Yu‐Xin
Kong, Yong
Yang, Fan
Zheng, Zhe
Hu, Xin‐Yue
Guo, Dong‐Sheng
Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
title Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
title_full Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
title_fullStr Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
title_full_unstemmed Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
title_short Supramolecular Tandem Assay for Pyridoxal‐5′‐phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein
title_sort supramolecular tandem assay for pyridoxal‐5′‐phosphate by the reporter pair of guanidinocalix[5]arene and fluorescein
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011187/
https://www.ncbi.nlm.nih.gov/pubmed/32071829
http://dx.doi.org/10.1002/open.201900316
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