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Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC

Context: Plantain fruit pulp has been used as a natural remedy to manage erectile dysfunction (ED) in traditional medicine. However, the potency of the peel has not been examined with respect to ED management. Objective: This study investigated and compared the inhibitory potential of unripe (UPP) a...

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Autores principales: Oboh, Ganiyu, Ademiluyi, Adedayo Oluwaseun, Oyeleye, Sunday Idowu, Olasehinde, Tosin Abiola, Boligon, Aline Augusti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011878/
https://www.ncbi.nlm.nih.gov/pubmed/28651482
http://dx.doi.org/10.1080/13880209.2017.1340966
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author Oboh, Ganiyu
Ademiluyi, Adedayo Oluwaseun
Oyeleye, Sunday Idowu
Olasehinde, Tosin Abiola
Boligon, Aline Augusti
author_facet Oboh, Ganiyu
Ademiluyi, Adedayo Oluwaseun
Oyeleye, Sunday Idowu
Olasehinde, Tosin Abiola
Boligon, Aline Augusti
author_sort Oboh, Ganiyu
collection PubMed
description Context: Plantain fruit pulp has been used as a natural remedy to manage erectile dysfunction (ED) in traditional medicine. However, the potency of the peel has not been examined with respect to ED management. Objective: This study investigated and compared the inhibitory potential of unripe (UPP) and ripe (RPP) plantain peels on some enzymes associated with ED and Fe(2+)-induced oxidative stress in albino rat penile homogenate in vitro. Materials and method: Aqueous extract of the peels was prepared and the effect on phosphodiesterase-5 (PDE-5), arginase, acetylcholinesterase (AChE), angiotensin-I converting enzyme (ACE) and Fe(2+)-induced malonyladehyde in isolated albino rat penile homogenate were investigated. Phenolic constituents of the peels powder were characterized using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Result: Extract from UPP had higher PDE-5 (IC(50) = 3.10 μg/mL), arginase (IC(50) = 0.96 μg/mL), AChE (IC(50) = 6.30 μg/mL) and ACE (IC(50) = 0.41 μg/mL) inhibitory ability compared with RPP (PDE-5, IC(50) = 4.33 μg/mL; arginase, IC(50) = 1.34 μg/mL; AChE, IC(50) = 8.64 μg/mL; ACE, IC(50) = 0.63 μg/mL). The extract from UPP also had higher inhibition of Fe(2+)-induced lipid peroxidation. HPLC-DAD analysis revealed that gallic and caffeic acids, rutin, quercitrin and quercetin were abundant in UPP, while catechin, kaempferol, chlorogenic and ellagic acids were the dominant phenolic compounds in RPP. Discussion and conclusion: Inhibition of enzymes associated with ED and lipid peroxidation could be linked with the phenolic compounds. However, UPP appeared to be more potent.
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spelling pubmed-70118782020-02-24 Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC Oboh, Ganiyu Ademiluyi, Adedayo Oluwaseun Oyeleye, Sunday Idowu Olasehinde, Tosin Abiola Boligon, Aline Augusti Pharm Biol Research Article Context: Plantain fruit pulp has been used as a natural remedy to manage erectile dysfunction (ED) in traditional medicine. However, the potency of the peel has not been examined with respect to ED management. Objective: This study investigated and compared the inhibitory potential of unripe (UPP) and ripe (RPP) plantain peels on some enzymes associated with ED and Fe(2+)-induced oxidative stress in albino rat penile homogenate in vitro. Materials and method: Aqueous extract of the peels was prepared and the effect on phosphodiesterase-5 (PDE-5), arginase, acetylcholinesterase (AChE), angiotensin-I converting enzyme (ACE) and Fe(2+)-induced malonyladehyde in isolated albino rat penile homogenate were investigated. Phenolic constituents of the peels powder were characterized using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Result: Extract from UPP had higher PDE-5 (IC(50) = 3.10 μg/mL), arginase (IC(50) = 0.96 μg/mL), AChE (IC(50) = 6.30 μg/mL) and ACE (IC(50) = 0.41 μg/mL) inhibitory ability compared with RPP (PDE-5, IC(50) = 4.33 μg/mL; arginase, IC(50) = 1.34 μg/mL; AChE, IC(50) = 8.64 μg/mL; ACE, IC(50) = 0.63 μg/mL). The extract from UPP also had higher inhibition of Fe(2+)-induced lipid peroxidation. HPLC-DAD analysis revealed that gallic and caffeic acids, rutin, quercitrin and quercetin were abundant in UPP, while catechin, kaempferol, chlorogenic and ellagic acids were the dominant phenolic compounds in RPP. Discussion and conclusion: Inhibition of enzymes associated with ED and lipid peroxidation could be linked with the phenolic compounds. However, UPP appeared to be more potent. Taylor & Francis 2017-06-27 /pmc/articles/PMC7011878/ /pubmed/28651482 http://dx.doi.org/10.1080/13880209.2017.1340966 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Oboh, Ganiyu
Ademiluyi, Adedayo Oluwaseun
Oyeleye, Sunday Idowu
Olasehinde, Tosin Abiola
Boligon, Aline Augusti
Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC
title Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC
title_full Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC
title_fullStr Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC
title_full_unstemmed Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC
title_short Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC
title_sort modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using hplc
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7011878/
https://www.ncbi.nlm.nih.gov/pubmed/28651482
http://dx.doi.org/10.1080/13880209.2017.1340966
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